低温保存肝脏糖原含量对肝脏微循环功能的保护作用.pdfVIP

· 146· 四川医学2008年2月第29卷(第2期)Sichu~mMedical Journal，2008，Vo／,29，No．2 论著 低温保存肝脏糖原含量对肝脏微循环功能的保护作用 汤礼军，田伏洲，汪 涛，黎冬喧，石 力，邹 树 (成都军区总医院全军普外中心，四川成都610083) 【摘要】 目的 探讨低温保存供肝糖原含量与肝脏微循环功能的关系及意义。方法 以兔为实验对象，采用术前 24h禁食、普食及普食加静脉注射葡萄糖等方法制备4组糖原含量不同(PO．05)的供肝模型。应用微循环多普勒血流 仪测定4组供肝血流再通后3o、6o及120mln时的血流量情况，并对供肝血流再通120rain时肝功能状况进行了检测。结 果 4组供肝于各时相，其微循环血流量值均表现为D组C组B组A组，且各组彼此间差异有统计学意义(P 0．01)。供肝再灌注120rain时，4组受体兔血液中AST、A工T及AKP含量变化均表现为A组B组C组D组，且各组彼 此间差异均有统计学意义(P0．05)。结论 在供肝切取前增加肝细胞糖原含量能显著改善再灌注期间肝脏微循环血 流量。 【关键词】肝脏；微循环；糖原 【中图分类号】R 331．3 5 【文献标识码】A 【文章编号】 1004-0501(2008)02．0146-02 Protection ofhelmtoeelldar#yeogen ORliver mi~ tionfUllt~doll auanghepatic cold pI 辩rva 0n．TANG ，TIAN Fu-zhou，WANG Tao，甜 ．Chengdu mili~ general hospital，Chengdu，S／chuan 610083，China 【Abstract】 Objective To study relationship between hepatocellular glycogen contents and hepatic microcirculafion function of donorliverduring cold preservationanditsmechanism．Methods Rat donorlivermodelswith different contents of glycogenwere estab- lished and the rates were divided into 4 groups(A，B，C and D)with abrosia for 24 hours before operation or common food or intravenous i@~tion sluea~e．The blood flow ofthe livers were measured by the microcirculation Doppler flow imaging after mperfusion for 30 rain- utes，60 minutes and 120 minutes，and the liver function ofthe receptor were measured after mpeffusion 120 minutes．Results The vol- uII1e ofthe donor livers microcirculafion blood flow display DCBA at each time phase-there was difference in the blood flow all the groups，the blood AST，AI and AKP contents of each receptor display ABCD，there was difference in each groups．Condu- sion To increase the glycogen of donator live