供试品种的遗传多样性与籼型水稻优良恢复系回交导入改良.pdfVIP

分子植物育种，2008年，第6卷，第1期，第25-31页 Molecular Plant Breeding，2008，Vo1．6，No．1，25—3 1 研究论文 Research Article 供试品种的遗传多样性与籼型水稻优良恢复系回交导入改良 崔金腾 陈冰蠕 石英尧 张蓉 王辉 钱益亮z刘海燕-朱苓华 黎志康 高用明- 1中国农业科学院作物科学研究所，北京，100081；2安徽农业大学农学院，合肥，230036；3中国农业科学院饲料研究所，北京，100081 通讯作者，gaoym@caas．net．on 摘 要 选取53个SSR标记，分析了55份参试水稻种质的遗传多样性和亲缘关系，以期为选配组合与改 良亲本提供参考。本试验共检测到267个等位变异，平均每个位点等位变异数为5．O4个，变化范围为4~7 个；53个SSR标记的多样性指数(PIC)平均为O．624，变化范围是0．287-4)．786。55份不同材料大体上可分为 籼稻和粳稻两大类，各品种间遗传距离在O．588-4)．996之间。明恢86与53份供体亲本间的相似性系数为 O．655-4)．850，蜀恢527与53份供体亲本间的相似性系数为O．640-4)．873。试验结果表明检测SSR多态性是 研究水稻品种之间遗传差异的高效、准确的手段之一，为高代回交导入群体的有利基因发掘和利用提供了有 益的参考。 关键词 水稻，SSR遗传多样性，相似性系数 Genetic Diversity of Involved Varieties and Improvement of Elite Restorer of Indica Rice Using Backcross Introgression Cui Jinteng Chen Bingxu Shi Yingyao Zhang Rong Wang Hui Qian Yiliang Liu Haiyan Zhu Linghua Li Zhikang Gao Yongming 1 Institute ofCrop Sciences，Chinesc Academy ofAgricultural Science，Beijing，100081；2 College ofAgricultural Sciences，Anhul Agricultural Uni— vemi~,Hefei，230036；3 Feed Research Institute，Chillese Academy ofAgricultural Science，Bcijing，100081 Correspondingauthor,gaoym@caas．net．cn Abstract A total of 53 SSR markers were used to analyze the genetic diversity and relationship among 55 rice germplasms for making reference to cross combinations and improvement of parents．In present study，267 allelic variation were detected,and the average allelic variation of53 loci is 5．04．ranged from 4 to 7．The average poly- morphism index content(PIE)of SSR markers is 0．624．ranged from 0．287 to 0．786．An germplasms could be di- vided into indica and iaponica rices，the genetic distances between them varied from 0．588 to 0．996．The compara． bility coe珩cient between Minghui 86 and 53 donors is from 0．655 to 0．850．it is from 0．64O to 0．873 between Shuhui 527 and the donors．The results indicated that detection of SSR polymorphisms was one of the most e珩． cient and accurate measures to studythe genetic di