益肾调督针灸法对AD大鼠海马线粒体CypD的影响.PDFVIP

- P P Effect of Yishen Tiaodu acupuncture-moxibustion on CypD of hippocampus mitochondria in Alzheimer’s disease rats CUI Xiang, MA Ran, TANG Qi, LI Dan, DU Yan-jun, SHEN Feng, SUN Guo-jie ( Advisor: QI Feng-jun ) ( Hubei University of Traditional Chinese Medicine, Wuhan 430065, China ) Abstract: Objective: To observe the effect of Yishen Tiaodu acupuncture-moxibustion on CypD of hippocampus mitochondria in Alzheimer’s disease (AD) rats, and to investigate the specii c mechanism of prevention-treatment of AD by acupuncture-moxibustion. Methods: Wistar rats were randomly divided into 4 groups: the normal group, sham-operated group, model group, and acupuncture-moxibustion group. First, bilateral hippocampus were injected A1-42 to make model of AD rats. The acupuncture-moxibustion group selected Baihui points (GV 20) and Shenshu points (BL 23), treated with acupuncture and moxibustion. The expression level of CypD of hippocampus mitochondria in rats was observed by immunohistochemistry method. Results: Compared with normal group and sham-operated group, the expression of CypD in model group was obviously higher than that of them (P0.01). Compared with the model group, CypD experssion of acupuncture-moxibustion group’ hippocampus mitochondria decreased and had Statistically signii cance (P0.01). Conclusion: Yishen Tiaodu acupuncture-moxibustion could ef ectively inhibit the expression of CypD of hippocampus mitochondria, blocking the formation of mitochondrial permeability transition pore, and decreasing mitochondrial damage. Thereby it will play a role in the treatment and prevention for AD. Key words: Yishen Ti