西尼罗病毒Chin_01株5_非编码区_UTR_中复制相关元件的功能研究.pdfVIP

# 816 # 2008 7 33 7 Med J Chin PLA V l 33 N 7 July 2008 西尼罗病毒Chin-01株5c非编码区(UTR)中复制相关元件的功能研究 李晓峰 姜涛 陈水平 韩剑峰 邓永强 秦成峰 于曼 秦鄂德 = Chin-015c( UT R) , , Chin-015cUT R , , , RdRp , NS5 RdRp , cDNA , 5cUT R 45- 60 , NS5 8 9 RNA NS5 , 8969 70 RNA, NS5 , 45- 60 8 9 Chin-015cUT R 45- 60 8969 70 = ; 5c; = R3731 3 Studies on thefunction of elements involvedinviralreplicationin5c untranslatedregion of WestNilevirus strainChin-01 Li Xia feng, Jiang Ta , Chen Shuiping, et al1 State Key Lab rat ry f Path gen and Bi security, Institute f Micr bi l gy and Epidemi l gy, Academy f Military Medical Sciences, Beijing 100071, China = bstract Object T study the functi ns f elements inv lved in viral replicati n in the 5c untranslated regi n ( 5cUT R) f West Nile virus ( WNV) strain Chin-01, s as t further clarify the m lecular details f viral replicati n. Methods A bi inf rmatic analysis f 5c UT R f strain Chin-01w as perf rmed and several nucle tides were selected as mutati ns intr duced int the 5cUT R f WNV. Then RdRp activity f NS5p l and NS5 using mutant 5cUT Rs as templates and capability f binding NS5 t mutant templates were bserved by RdRp assay in v itr o and EMSA, respectively. Finally, the mutati ns in elements inv lved in viral replicati n were intr duced int the infecti us ful-l length cDNA cl ne f strain Chin-01. Then the bi l gical characteristics f the mutant rec vered virus were bserved. Results Dele- ti n f nucle tides 46- 60 in 5cUTR resulted in deficiency f RdRp activity f NS5p l and NS5F, while mutati ns at nucle tides 8 and 9 re- sulted in deficiency f RdRp activity and capability f binding t templates f tw pr teins using mutants c ntaining 5cUT R as templates. H wever, nly the mutant with mutati ns at nucle tides 8,