养心通脉有效部位方促血管生成作用实验探究.doc

养心通脉有效部位方促血管生成作用实验探究摘 要:目的;探讨养心通脉有效成分部位方(YTⅡ)促进血管新生,治疗心肌缺血的机理。方法:运用血清药理学的方法制备含药血清,鸡胚随机分为养心通脉方(YTⅠ)组、养心通脉有效部位方(YTⅡ)组、bFGF组和空白血清(KX)组;72h后观察比较YTⅡ及各含药血清对各组CAM模型鸡胚存活情况、CAM标本形态、CAM血管数量的影响。结果:加药72h后,各药物组与空白血清组比较皆有统计学意义(均PYTⅡ组YTⅠ组KX组趋势。结论:YTⅡ与YTⅠ、bFGF一样有促血管新生作用,且YTⅡ作用与bFGF作用相当,明显优于YTⅠ。 关键词:养心通脉有效部位方(YTⅡ);血管生成 ; 鸡胚绒毛尿囊膜 中图分类号:R285.5 文献标识码:A 文章编号:1673-7717(2010)01-0054-03 Positive Research on the Effective Components Recipe of YangXinTongMai Formula(YT II) on the Angiogenesis MO Li,YUAN Zhao?kai , HUANG Xian?ping , CHEN Qing?hua , JIAN Wei?xiong,HU Zhi?xi,SUN Gui?xiang (Hunan University of TCM, Changsha 410007,Hunan, China) Abstract:Objective: To investigate the mechanism of the effective components recipe of YangXinTongMai Formula on the angiogenesis and improving myocardial ischemia. Methods:The chorioallantoic membrane(CAM) model was cultured and devided into YangXinTongMai Formula(YT I )group and controlled group including the effective components recipe of YangXinTongMai Formula(YT II) group, bFGF group and KX group.the survival rate,growth shape and vessel counts of CAM model in every group was observed after 72 hours. Results:The vessel counts in bFGF group,YT-II group and YT-I group is more than KX group (PYT-II groupYT-I groupKXgroup. Conclusion: Our study indicated that YT-II can improving angiogenesis as well as YT-I and bFGF,and more better than YT-I to some extent. Key words:the effective components recipe of YangXinTongMai Formula(YT II) ; angiogenesis; chorioallantoic membrane 冠心病是冠状动脉狭窄或闭塞导致心肌缺血,从而影响心功能的临床常见心血管疾病。近年来冠状动脉旁路移植术、经皮冠脉内血管成形术等新技术的开展取得了一定疗效,但仍有相当部分病人药物治疗效果不佳且并不适宜血管重建术,因此通过治疗性血管增生以减轻心肌缺血逐渐成为目前研究的热点。养心通脉方(YTⅠ)是根据秦伯未先生“扶养心气,和通血脉”之法制定的治疗胸痹心痛的有效名方,由人参、丹参、枳实、泽泻、桂枝等按一定比例组成,对缺血性心脏病、高脂血症、动脉粥样硬化等均具有显著的临床疗效[1-2]。养心通脉有效部位方(YTⅡ)是笔者从YTⅠ原方综合提取出的人参皂苷等10个成分部位中,通过前期系列实验所确定的“最佳有效成分部位方”。本项研究主要通过观察药物作用72h后,鸡胚绒毛尿囊膜模型(chorioallantoic membrane,CAM)的血管形态学和新生血管计数的变化,以探讨养心通脉有效成分部位方(YTⅡ)促进血管新生,治疗心肌缺血的机理。 1材料与方法 1.1实验材料 1.1.1 实验动物 清洁级健康雄性SD大鼠