氟伐他汀对兔动脉内膜损伤后血管平滑肌细胞增殖影响.docVIP

氟伐他汀对兔动脉内膜损伤后血管平滑肌细胞增殖影响摘 要：目的：探讨氟伐他汀对兔动脉内膜损伤后血管平滑肌细胞(VSMCs)增殖的影响。方法：将20只新西兰兔随机分为正常组(n=6)、模型组(n=7)、氟伐他汀组(n=7)。通过高脂饲料配合腹主动脉内膜剥脱术建立兔动脉内膜损伤模型。取腹主动脉下段，电镜观察血管平滑肌细胞形态；应用RT-PCR法测定原癌基因c-myc和血小板源性生长因子-BB(PDGF-BB)水平；免疫组化法测定胰岛素样生长因子-1(IGF-1)水平。结果：氟伐他汀组与模型组相比血管平滑肌细胞增殖水平明显减低；PDGF-BB和IGF-1水平显著降低(P0.05)；c-myc和PDGF-BB mRNA表达的相关性分析显示二者呈正相关，模型组相关系数为8.603(P0.05)，氟伐他汀组相关系数为2.467(P0.05)；而IGF-1与c-myc无相关性。结论：氟伐他汀通过抑制PDGF和IGF-1的合成和分泌抑制VSMCs增殖，这种作用部分是通过抑制c-myc表达实现的。 关键词：氟伐他汀；血管平滑肌细胞；血小板源性生长因子；胰岛素样生长因子-1；原癌基因 中图分类号：R-33 文献标识码：A 文章编号：1673-7717(2008)03-0556-03 The Effect of Fluvastatin on Proliferation of Vascular Smooth Muscle Cells in Rabbits after Endarterium Injury QI Xiao-yun，YANG Guan-lin，LIN Ping，SHANG Nan，GAO Min，ZHANG Si-wei (1.Liaoning University of Traditional Chinese Medicine，Shenyang 110032，Liaoning，China) Abstract：Objective：To investigate the effect of fluvastatin on vascular smooth muscle cells (VSMCs) proliferation in rabbits after endarterium injury.Methods：Twenty New Zealand rabbits were randomly divided into normal group(n=6)，model group(n=7)and Fluvastatin control group(n=7). Through feeding rabbits with high dosage of cholesterol diet along with endarterium denudation，rabbits endarterium-injured models were build. Vascular smooth muscle cells (VSMCs) were observed under the electron microscope. Reversetranscription-polymerase chain reation (RT-PCR) method was used for observing the levels of c-myc and platelet derived growth factor-BB(PDGF-BB). The level of Insulin-like growth factor-1 (IGF-1) was collected by immunohisto chemistry. Results：The proliferation level of VSMCs in fluvastatin control group was lower than that of the model group. The levels of PDGF-BB，c-myc and IGF-1 in model group were markedly higher than those of the normal group (P0.01)；The levels of PDGF-BB and IGF-1 in fluvastatin control group were much lower than those of model group (P0.05)；The levels of PDGF-BB，c-myc and IGF-1 in fluvastatin group were much lower than those of m