柴胡疏肝散对慢性应激抑郁模型大鼠行为及血浆促肾上腺皮质激素释放激素和促肾上腺皮质激素的影响.docVIP

　　柴胡疏肝散对慢性应激抑郁模型大鼠行为及血浆促肾上腺皮质激素释放激素和促肾上腺皮质激素的影响 ：李云辉, 张春虎, 王素娥, 邱娟, 胡随瑜, 肖桂林 【摘要】 目的：探讨柴胡疏肝散对慢性应激抑郁模型大鼠行为学及血浆促肾上腺皮质激素释放激素（corticotropin releasing hormone，CRH）和促肾上腺皮质激素（adrenocorticotropic hormone，ACTH）浓度的影响。方法：40只雄性SD大鼠随机分为空白对照组、模型组、氟西汀组及柴胡疏肝散组。采用慢性轻度不可预见性应激加孤养复制大鼠抑郁模型（4周）。所有大鼠在造模第15天开始灌胃，分别灌服等体积生理盐水（空白对照组、模型组）或氟西汀（1.8 mg/kg）及柴胡疏肝散（5.9 g/kg）药液，连续2周。选用敞箱实验得分和液体消耗量等指标评价各组大鼠行为学改变，采用放射免疫法检测各组血浆CRH和ACTH浓度。结果：与空白对照组比，模型组大鼠体质量增加缓慢，敞箱实验中的水平运动、垂直运动得分、清洁动作次数显著减少，中央格停留时间显著延长，糖水消耗明显下降，纯水消耗显著增多，血浆CRH和ACTH浓度增高, 差异有统计学意义（P＜0.05）。柴胡疏肝散组大鼠抑郁样行为显著改善，血浆CRH和ACTH浓度较模型组显著降低，差异有统计学意义（P＜0.01）。结论：慢性轻度不可预见性应激可使大鼠行为及神经内分泌发生异常改变，引起抑郁；柴胡疏肝散可调节慢性应激引起的HPA轴功能亢进，具有抗抑郁作用。 【关键词】 柴胡疏肝散; 抑郁; 慢性应激; 神经行为学表现; 促肾上腺皮质激素释放激素; 促肾上腺皮质激素; 大鼠 　　Objective: To investigate the effects of Chaihu Shugan San (CHSGS), a pound traditional Chinese herbal medicine, on behavior and plasma levels of corticotropin releasing hormone (CRH) and adrenocorticotropic hormone (ACTH) of rats ild unpredicted stress depression. 　　Methods: Forty male SpragueDaly divided into 4 groups: normal control group, untreated group, fluoxetine group and CHSGS group. Except the normal control group, rats ild stressor for continuous 4 inistered e agents respectively for 2 al saline for the normal control group and the untreated group, fluoxetine (1.8 mg/kg) for the fluoxetine group and CHSGS (5.9 g/kg) for the CHSGS group］. Behavioral scores of rats a levels of CRH and ACTH in different groups munoassay. 　　Results: pared al control group, body ing in openfield test ption in sucrose preference test a CRH and ACTH proved significantly and the levels of plasma CRH and ACTH ild unpredicted mild stress can affect the neuroendocrine and behavior and cause depression in rats. CHSGS can regulate HPA hyperactivity of rats caused by chronic stress and has antidepressive effects. 　　Keyanifestations; corticotropin releasing hormone; adrenocorticotropic hormone; rats 　　抑郁症是最常见的心境障碍，普通人群中抑郁症的年发病率约为200/10万，且发病率呈逐年上升趋势。据世界卫生组织估计，2020年抑郁症将成为第二大疾病［1］。抑郁症属中医