老龄小鼠胸腺内皮细胞的变化.pdfVIP

解剖科学进展 Progress of Anatomical Sciences 2008 ,14(1):24-27,31 老龄小鼠胸腺内皮细胞的变化 朱喜科1 ，王振字，鲁阳1 (中国医科大学1.盛京医院实验研究中心;2 基础医学院解剖学教研室，辽宁沈阳 IIαlOl ) {摘要]目的研究老化引起胸腺萎缩和功能减低的相关机制。方法 用组织化学和免疫荧光染色方法分析 胸腺皮质和髓质结构;用BrdU标记流式细胞仪方法研究胸腺内皮细胞增殖;细胞凋亡测定用TUNNEL流式细胞仪 方法。结果 老龄小鼠胸腺内皮细胞增殖阳性率6.70，品，对照年轻小鼠为9.20，品。老龄小鼠胸腺内皮细胞凋亡率为 1 1.30，品，对照年轻小鼠为2.6%。老龄小鼠胸腺结掏出现退行性改变。结论 老化引起胸腺内皮细胞增殖能力下 降、凋亡率增加和胸腺结掏出现退行性改变，可能是老年胸腺萎缩免疫力降低的原因之一。 {关键词]老化;胸腺萎缩;胸腺内皮细胞 {中图分类号 1 R322S3 [文献标识码 1 A [文章编号 1 1 ∞6-2947 (2∞8) 01-∞24-ω The Abnormal Changes of the Thymic Epithelial Cells Caused by Aging 1 2 1 ZHU Xi-ke ,WANG Zhen-yu ,LU Yang (1. Core-Facility for Medic温1 Research , Shengjing Ho电 pital; 2. Department of Anatomy , Basic Medical College, China Medical University, Liaoning Shenyang 11αlO l China) [ Abstract 1 Objective To study the mechanism of age-related thymic involution. Methods The architecture of thymic cortico-medullary was analyzed by histochemistry and immunofluorescence staining and the proliferation and apoptosis of thymic 叩ithelial cells (TECs) was determinated by BrdU-flowcytometry and TUNEL-flowcytometry respectively. Results The positive rate of old mice TECs proliferation was 6.7% and that of control group of young mice was 9.20，品. The apoptosis rate of old mice TECs was 11.30，毛 and that of control group of young mice was 2.6%. Thymic architecture involution occurred in aging. Conclusion The aging causes the decrease of TEC proliferation ability , the increase of TEC apoptosis rate and the thymic involution , which may be one of the reasons of the thymic atrophy and the df~rease of immunity in aging. [ Key words 1 aging; thymic atrophy; thymic epithe