CT灌注成像对大鼠急性脑缺血及再灌注模型的评价.doc

CT灌注成像对大鼠急性脑缺血及再灌注模型的评价 马秀华，吕富荣,吕发金,肖智博,黄铀新，李新友,杨 柳,潘 静 （重庆医科大学附属第一医院放射科，重庆 400016） 摘 要：目的 在活体状态下应用CT灌注成像（CT perfusion imaging,CTPI）技术，评价大鼠急性脑缺血及再灌注模型的可靠性及准确性。方法 用改良线栓法制作大鼠急性脑缺血及再灌注模型，模型分别行CTPI检查、2,3,5-三苯基氯化四氮唑（TTC）染色及HE染色。结果 ①每组CTPI各时间点脑血流量（cerebral blood flow,CBF）图核心区及脑血容量（cerebral blood volume, CBV）图低灌注区体积之间无统计学差异（P＞0.05）,两者所示体积与TTC染色梗死区体积之间无统计学差异（P＞0.05）,与平均通过时间（mean transit time,MTT）图缺血区体积之间有统计学差异（P＜0.05）。MTT缺血区体积与TTC染色梗死区体积之间有统计学差异（P＜0.05）。② CTPI各参数图与TTC染色梗死体积间均呈正相关关系，其中CBV相关性最好（r=0.74）。③梗死区CBF、CBV较对侧均明显减低，MTT延长；缺血半暗带区(Ischemic penumbra ,IP)CBF较对侧明显减低，CBV较对侧正常或升高，MTT轻度延长；周围相对正常区CBF、CBV及MTT较对侧未见明显改变。④取梗死区脑组织做HE染色,结果显示以神经细胞死亡表现为主；取IP区脑组织做HE染色，显示以神经细胞变性损伤为主。结论 CTPI可在活体状态下实现快速、准确、无创的评价脑缺血再灌注动物模型的脑部血流动力学改变，显示脑梗死区及缺血半暗带区的部位、大小、程度，对超急性期脑梗死治疗及复查具有重要的临床应用价值。 关键词∶CT灌注成像；急性脑缺血；脑部血流动力学；半暗带；大鼠 中图法分类号： 文献标识码： Evaluating rat model of acute cerebral ischemia-reperfusion by computed tomography perfusion imaging MA Xiu-hua, LU Fu-rong, LU Fa-jin, XIAO Zhi-bo, HUANG You-xin, LI Xin-you, YANG Liu, PAN Jing (Department of Radiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China) Abstract: Objective To evaluate the reliability and accuracy of rat model of acute cerebral ischemia-reperfusion in living condition by using CT perfusion imaging (CTPI) technology. Methods A stable and reversible acute cerebral ischemia-reperfusion model with unilateral middle cerebral occlusion was established in Wistar rats and evaluated by CTPI, TTC staining and HE staining. There were two groups: ischemia group and ischemia+reperfusion group. Results ①Cerebral blood flow (CBF) core areas and cerebral blood volume (CBV) of low volume infusion areas on CTPI showed no significant difference at various time points and between the two groups (P0.05), and the results obtained from CTPI were concordant with those from TTC staining for infarction zone (P0.05), but significantly different with the ischemic volume of average transit time (MTT) (P0.05).