高效液相色谱法测定伊维菌素脂质体药物的含量及包封率_佘永新.pdfVIP

·80 · 　2010 37 4 1 1 1 3 2 1 1 1 , , , , , , , (1., 　850009;2. , 　100081;3., 　464000) :。Waters surfire C18 (150 mm× 2.1 mm, 5 μm), — —(62 ∶30 ∶8), 1 mL/min, 245 nm, 10 μL, 30 ℃。 、、、 4 。 , , 1～ 50 μg/mL (r=0.9998, n=5), , 98.54%±1.6%。 、, 。 :;;;; :S859.83　　　　　:A　　　　　:1671-7236(2010)04-0080-05 　　(Ivermectin, IVM) 12 、73 , , 、 , , 、、、(Aránzazu , IVM 3 (、、)、 2009; mura, 2008;Mckellar , 1996;Hans , 2007)。(、、、 :2009-11-13 、、)、 :(1972-), , , , , , , : 。 , 。 :(O10-1)。 10 Zsak L, Borca M V.Preclinical diagnosis of African swine fever Clinical Microbiology, 2005, 43(1):112～ 119. in contact-exposed swine by a real-time PCR assay [J] .Journal of Research and Assessment of Real-time Quantitative PCR Assay for the Detection of African Swine Fever Virus 1, 2 2 2 GUO Shao-ping , LIU Jian , WU Shao-qiang (1.Inner Mongolia Agricultural University, Hohhot 010018, China; 2.Chinese Academy of Inspection and Quarantine, Beijing 100029, China) 　　Abstract:A real-time quantitative PCR(qPCR)assay for the detection of African swine fever virus(ASFV)has been devel- oped by designing the primers and TaqMan probe according to the sequence of the geneK 205R .The reaction solutions and con- ditions were optimized by using different concentrations of the primers and Mg2+ and different annealing temperature.The mask samples were adopted to verify its actual detective efficiency by adding the plasmid D A into swine liver tissue.The qPCR detective metho