鸡Myostatin基因单核苷酸多态性及其对屠体性状的遗传效应分析.pdfVIP

HEREDITAS (Beijing) 2007 5 , 29(5): 593598 ISSN 0253-9772 DOI: 10.1360/yc-007-0593 Myostatin 1 1 2 , , 1. , 625014; 2. , 310029 : 180 3 , PCR-RFLPMSTN 1 2 , Bsh 1236 G(2100)A, MN NN 2 , Msp G(2109)A, AA AB BB 3 , 2 5 χ2 (P 0.05)(P 0.01 P 0.05)MN (P 0.05)NN ; AB (P 0.01 P 0.05 )AA , AA (P 0.01)BB , , BB (P 0.05)AA AB 2 , NA/MA (P 0.01) : ; Myostatin ; RFLP; ; SNPs of Myostatin gene and its genetic effects on carcass traits in chicken 1 1 2 ZHU Zhi , WU Deng-Jun , XU Ning-Ying 1. College of Animal Science and Technology, Sichuan Agricultural University, Ya’an 625014, China; 2. College of Animal Science, Zhejiang University, Hangzhou 310029, China Abstract: Two polymorphic sites in the first exon 1 of MSTN gene in 180 Wenling grass-chicken including 3 lines were studied by PCR-RFLP method,and its genetic effects on carcass traits were researched. The results showed that 2 genotypes (MN and NN) in G(2100)A locus were identified by Bsh 1236 and 3 genotypes (AA , AB and BB) in G(2109)A locus were identified by Msp , and 5 genotypes were emerged while com-analyzing 2 loci. The χ2 test indicated genotype frequencies had no significant deviation (P 0.05) among lines. The analysis of variance showed that the different genotypes had sig- nificant deviation (P 0.01 or P 0.05) in carcass percent. The multiple comparisons were made. The abdominal fat weight and carcass percent of heterozygosis MN were significantly higher (P0.05) than that of mut