遗传工程无籽果实原理及其研究进展.pdfVIP

( ) 浙江农业学报 Acta Agriculturae Zhejiangensis 15 6 :365～371 , 2003 　　　　　　　　　　　　　　　　　　　　　　　 遗传工程无籽果实原理及其研究进展 (浙江省农业科学院 园艺研究所 ,浙江 杭州 310021) 陈俊伟 ,谢 　鸣 , 吴 　江 　 摘 　要 : 无籽是一个优良的果实性状 。用杂交 、芽变 、化学或物理因子诱变 、三倍体育种等传统途 径选育无籽果实品种 ,具有选育周期长 ,且极少与优 良性状连锁的缺点 。应用植物生长调节剂处 理也可诱导无籽果实 ,但使用技术难以掌握并受气候制约 ,还会带来食物安全问题 。采用遗传工 程技术将调控无籽形成的激素代谢与信号转导基因、花器官发育基因及细胞毒素基因转入有籽的 优良品种中 ,可创造出优质无籽新品种 。 关键词 : 无籽果实 ; 单性结实 ;遗传工程 ( ) 中图分类号 : S603. 2 　　　　　　文献标识码 : A 文章编号 :1004 - 1524 2003 06 - 0365 - 07 The principle of breeding of seedless fruit by genetic engineering and its study advance :CHEN Junwei , XIE Ming , WU Jiang ( Institute of Horticulture , Zhejiang Academy of Agricultural Sciences , Hangzhou 310021 , ) China Abstract : Seedlessness is a desirable trait in fruits. Traditional seedless fruit varieties were obtained by hy bridization , bud mutation , chemical and physical factor mutation and triploid methods. But the drawbacks of these methods are a long period for selection of a new variety and the seedlessness seldom link to the trait of good quality. Seedless fruits have also been induced by application of plant growth regulators ; however , this method is technically difficult and is affected by weather. The seedless fruits produced by the regulators are not safety. The seedless genes , i. e. regulate the metabolism and signal transduction of plant endogenous hormone or floral organ development or a cytotoxic gene , are introduced into high quality seedy varieties by genetic engi neering. After these genes expression under the direct of ovary specific promoter in the fruit , the high quality seedless va