黄体酮减轻大鼠蛛网膜下腔出血后早期脑损伤.docVIP

黄体酮减轻大鼠蛛网膜下腔出血后早期脑损伤 探讨黄体酮对蛛网膜下腔出血（subarachnoid hemorrhage SAH)后早期脑损伤（early brain injury EBI）细胞凋亡、血脑屏障（blood–brain barrier BBB）稳定性、脑水肿和病死率。方法 66只大鼠被随机分为假手术组，SAH+组，SAH+黄体酮组。SAH模型制成后1、6 h和12 h分别给予黄体酮（16/kg）或等体积的。在SAH24 h分析病死率神经功能评分脑水肿细胞凋亡caspase-3及基质金属蛋白酶-9（MMP-9表达。结果 与SAH+组比较，黄体酮治疗降低了病死率细胞凋亡caspase-3水平MMP-9的表达，脑水肿伊文思蓝渗出，提高了神经功能评分结论 黄体酮可通过抑制细胞凋亡和稳定BBB减轻SAH后EBI。关键词蛛网膜下腔出血早期脑损伤黄体酮细胞凋亡血-脑屏障Progesterone attenuates early brain injury after subarachnoid hemorrhage in rats YAN Yu-jing, CHEN Gao, WANG Lin, YAN Fen (Derpartment of Neurosurgery, the second affiliated hospital of Zhejiang university school of medicine, Hangzhou 310009, China) [Abstract] objective In this study, we explored the effect of Progesterone on cell apoptosis, stability of the blood–brain barrier (BBB), brain edema, and mortality in male Sprague-Dawley rats subjected to subarachnoid hemorrhage-induced EBI by endovascular Perforation. Methods Rats (n=66) were randomly assigned to sham, SAH+vehicle, and SAH+progesterone groups. Progesterone (16 mg/kg) or an equal volume of vehicle was administered at 1 h, 6 h and 12 h after SAH. Mortality within 24 h, neurological scores, brain edema, Evans blue dye extravasation, cell apoptosis, and the expression of casPase-3 and matrix metalloproteinase (MMP)-9 were assayed after 24 h of SAH. Results Progesterone treatment significantly reduced mortality, brain edema, Evans blue dye extravasation, cell apoptosis, expression of casPase-3 and MMP-9, and improved neurological scores compared with the vehicle group. Conclusions Progesterone may reduce EBI after SAH by inhibiting cell apoptosis and stabilizing the BBB. [Key words] Subarachnoid hemorrhage, Early brain injury, Progesterone, Apoptosis, Blood–brain barrier Supported by ministry of department of Zhejiang Province to build Project（WSJ29-2-025）.Corresponding author: CHEN Gao , E-mail: d.chengao@163.com 黄体酮对的神经保护作用已经得到了大量研究的支持[1]。黄体酮可有效的减少损伤后24h内的细胞死亡[]，降低组织损伤，改善创伤性脑损伤后行为[3]，稳定血脑屏障（blood–brain barrier BBB）和