expression of -alkylguanine-dna alkyltransferase in normal and malignant bladder tissue of egyptian patients正常和恶性膀胱组织中表达-alkylguanine-dna alkyltransferase埃及的病人.pdfVIP

SAGE-Hindawi Access to Research Journal of Nucleic Acids Volume 2010, Article ID 840230, 9 pages doi:10.4061/2010/840230 Research Article Expression of O6-Alkylguanine-DNA Alkyltransferase in Normal and Malignant Bladder Tissue of Egyptian Patients Abir A. Saad,1, 2 Heba Sh. Kassem,1, 3 Andrew C. Povey,4 and Geoffrey P. Margison1 1 Cancer Research UK Carcinogenesis Group, Paterson Institute for Cancer Research, The University of Manchester, Manchester M20 4BX, UK 2 Institute of Graduate Studies and Research, University of Alexandria, Alexandria 21526, Egypt 3 Pathology Department and Clinical Genomics Center, Faculty of Medicine, University of Alexandria, Alexandria 21526, Egypt 4 Centre for Occupational and Environmental Health, Faculty of Medical and Human Sciences, The University of Manchester, Manchester M13 9PL, UK Correspondence should be addressed to Geoffrey P. Margison, gmargison@picr.man.ac.uk Received 12 June 2010; Accepted 17 August 2010 Academic Editor: Ashis Basu Copyright © 2010 Abir A. Saad et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Bladder tumour tissues and corresponding uninvolved mucosa (normal tissue) of Egyptian bladder cancer patients were assessed for O6 -alkylguanine-DNA-alkyltransferase (MGMT) activity by functional assay of tissue extracts (36 paired samples), and distribution by immunofluorescence (IF) microscopy of fixed material (24 paired samples). MGMT varied widely from 42– 253 fmoles/mg protein and from 3.2–40 fmoles/µg DNA in normal and 58–468 fmoles/mg protein and 2.5–49.5 fmoles/mg protein, in the tumour tissues; only o