differential secondary reconstitution of in vivo-selected human scid-repopulating cells in nodscid versus nodscidγ chainnull mice微分二次调整在nodscid vivo-selected人类scid-repopulating细胞和nodscidγchainnull老鼠.pdfVIP

Hindawi Publishing Corporation Bone Marrow Research Volume 2011, Article ID 252953, 11 pages doi:10.1155/2011/252953 Research Article Differential Secondary Reconstitution of In Vivo -Selected Human SCID-Repopulating Cells in NOD/SCID versus NOD/SCID/γ chainnull Mice Shanbao Cai,1, 2 Haiyan Wang,1, 2 Barbara Bailey,1, 3 Jennifer R. Hartwell,1, 2 Jayne M. Silver,3 Beth E. Juliar,4 Anthony L. Sinn,3 Arthur R. Baluyut,5 and Karen E. Pollok1, 3 1 Section of Pediatric Hematology/Oncology, Department of Pediatrics, Herman B Wells Center for Pediatric Research, Indiana University Simon Cancer Center, The Riley Hospital for Children, 980 West Walnut Street, R3 516, Indianapolis, IN 46202-5525, USA 2 Indiana University Simon Cancer Center (IUSCC), Indiana University School of Medicine (IUSM), Indianapolis, IN 46202, USA 3 In Vivo Therapeutics Core, IUSCC, Indianapolis, IN 46202, USA 4 Biostatistics and Data Management Core, IUSCC, Indianapolis, IN 46202, USA 5 Northside Gastroenterology, St. Vincent Hospital, Indianapolis, IN 46260, USA Correspondence should be addressed to Karen E. Pollok, kpollok@ Received 2 August 2010; Revised 21 October 2010; Accepted 27 October 2010 Academic Editor: A. Ganser Copyright © 2011 Shanbao Cai et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Humanized bone-marrow xenograft models that can monitor the long-term impact of gene-therapy strategies will help facilitate evaluation of clinical utility. The ability of the murine bone-marrow microenvironment in NOD/SCID versus NOD/SCID/γ chainnull mice to support long-term engraftment of MGMTP140K -transdu