differential mechanisms of ang (1-7)-mediated vasodepressor effect in adult and aged candesartan-treated rats微分和机制(1 - 7)介导vasodepressor效应在成年和老年candesartan-treated老鼠.pdfVIP

Hindawi Publishing Corporation International Journal of Hypertension Volume 2012, Article ID 192567, 9 pages doi:10.1155/2012/192567 Research Article Differential Mechanisms of Ang (1-7)-Mediated Vasodepressor Effect in Adult and Aged Candesartan-Treated Rats S. Bosnyak,1 R. E. Widdop,1 K. M. Denton,2 and E. S. Jones1 1 Departments of Pharmacology, Monash University, Clayton, VIC 3800, Australia 2 Departments of Physiology, Monash University, Clayton, VIC 3800, Australia Correspondence should be addressed to R. E. Widdop, robert.widdop@ Received 29 July 2011; Accepted 7 September 2011 Academic Editor: Robson Santos Copyright © 2012 S. Bosnyak et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Angiotensin (1-7) (Ang (1-7)) causes vasodilator effects in Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHRs) via angiotensin type 2 receptors (AT R). However, the role of vascular AT R in aging is not known. Therefore, we examined the 2 2 effect of aging on Ang (1-7)-mediated vasodepressor effects and vascular angiotensin receptor localization in aging. Blood pressure was measured in conscious adult ( ∼17 weeks) and aged (∼19 months) normotensive rats that received drug combinations in a randomised fashion over a 4-day protocol: (i) Ang (1-7) alone, (ii) AT1R antagonist, candesartan, alone, (iii) Ang (1-7) and candesartan, or (iv) Ang-(1-7), candesartan, and the AT2 R antagonist, PD123319. In a separate group of animals, the specific MasR antagonist, A779, was administered in place of PD123319. Receptor localisation was