原创力文档autoantibodies profile in the sera of patients with sjogren]s syndrome the ana evaluation—a homogeneous, multiplexed system自身抗体在干燥)s综合症患者的血清ana evaluation-a均匀,多路复用系统.pdfVIP
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autoantibodies profile in the sera of patients with sjogren]s syndrome the ana evaluation—a homogeneous, multiplexed system自身抗体在干燥)s综合症患者的血清ana evaluation-a均匀,多路复用系统
Clinical Developmental Immunology, March 2004, Vol. 11 (1), pp. 53–56
Autoantibodies Profile in the Sera of Patients with Sjogren’s
Syndrome: The ANA Evaluation—A Homogeneous,
Multiplexed System
a b a, c
BORIS GILBURD , MAHMOUD ABU-SHAKRA , YEHUDA SHOENFELD *, ANDREA GIORDANO ,
ELENA BARTOLONI BOCCIc, FRANCESCO DELLE MONACHEc and ROBERTO GERLIc
aDepartment of Medicine B, Center for Autoimmune Diseases, Sheba Medical Center, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Hashomer,
Israel; bAutoimmune Rheumatic Disease Unit, Soroka Medical Center and Ben-Gurion University, Beer-Sheva, Israel; cSection of Internal Medicine
and Oncological Services, Department of Clinical and Experimental Medicine, Center for Study of Rheumatic Diseases, University of Perugia, Perugia,
Italy
Background : Flow-based, multiplex bead arrays (MBA) have been developed for a variety of
applications including the detection of antibodies to extractable nuclear antigens (ENA). It offers a
rapid and sensitive method to assess multiple analyses in a single tube/well.
Purpose: To evaluate the Athena Multi-Lyte ANA Test System utilizes Luminex Corporation’s
MBA technology for the detection of antinuclear antibodies (ANA) and ENA antibodies in the sera of
patients with Sjogren’s syndrome (SS).
Methods : MBA assay was used to detect ANA and ENA antibodies in the sera of 37 patients with SS
and 96 sera from healthy subjects.
Results : All patients were wome
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