biodegradation of melamine and cyanuric acid by a newly-isolated microbacterium strain生物降解的三聚氰胺和三聚氰酸newly-isolated细杆菌属菌株.pdfVIP
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biodegradation of melamine and cyanuric acid by a newly-isolated microbacterium strain生物降解的三聚氰胺和三聚氰酸newly-isolated细杆菌属菌株
Advances in Microbiology, 2012, 2, 303-309
/10.4236/aim.2012.23036 Published Online September 2012 (http://www.SciRP.org/journal/aim)
Biodegradation of Melamine and Cyanuric Acid by a
Newly-Isolated Microbacterium Strain
*
Naofumi Shiomi , Maiko Ako
School of Human Sciences, Kobe College, Nishinomiya, Japan
*
Email: n-shiomi@mail.kobe-c.ac.jp
Received April 25, 2012; revised May 28, 2012; accepted June 11, 2012
ABSTRACT
We searched for a superior melamine-degrading bacterium for the bioremediation of melamine. Cyanuric acid, which is
a by-product produced during the biodegradation of melamine, shows strong nephrotoxicity. Therefore, the mela-
mine-degrading bacterium is also required to show a high ability to degrade cyanuric acid. We selected a mela-
mine-degrading strain (MEL1) among ten cyanuric acid-degrading bacteria isolated from the soil. The species of MEL1
strain was Microbacterium esteramaticum or was extremely similar to that species, and the enzymatic activity of the
melamine deaminase in the MEL1 strain was similar to that in the NRRLB-12227 strain. The ability of the MEL1 strain
to degrade cyanuric acid was higher than its ability to degrade melamine, and therefore, the accumulation of by-prod-
ucts (ammeline, ammelide and cyanuric acid) during the degradation of melamine was minimal. These results suggest
that the MEL1 strain is useful for the bioremediation of melamine.
Keywords: Melamine; Cyanuric Acid; Melamine Deaminase; Microbacterium
1. Introduction made of th
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