characterization of plasmid ppo1 from the hyperacidophile picrophilus oshimae描述的质粒ppo1 hyperacidophile picrophilus oshimae.pdfVIP

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characterization of plasmid ppo1 from the hyperacidophile picrophilus oshimae描述的质粒ppo1 hyperacidophile picrophilus oshimae.pdf

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characterization of plasmid ppo1 from the hyperacidophile picrophilus oshimae描述的质粒ppo1 hyperacidophile picrophilus oshimae

Hindawi Publishing Corporation Archaea Volume 2011, Article ID 723604, 4 pages doi:10.1155/2011/723604 Research Article Characterization of Plasmid pPO1 from the Hyperacidophile Picrophilus oshimae 1 ¨ 2 1 Angel Angelov, Jorn Voss, and Wolfgang Liebl 1 Lehrstuhl fur Mikrobiologie, Technische Universitat Munchen, Emil-Ramann-Straße 4, Weihenstephan, 85354 Freising, Germany ¨ ¨ ¨ 2 Institut fur Mikrobiologie und Genetik, Georg-August-Universitat Gottingen, Grisebachstrasse 8, 37077 Gottingen, Germany ¨ ¨ ¨ ¨ Correspondence should be addressed to Wolfgang Liebl, wliebl@wzw.tum.de Received 1 June 2011; Accepted 21 July 2011 Academic Editor: Matthias Hess Copyright © 2011 Angel Angelov et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Picrophilus oshimae and Picrophilus torridus are free-living, moderately thermophilic and acidophilic organisms from the lineage of Euryarchaeota. With a pH optimum of growth at pH 0.7 and the ability to even withstand molar concentrations of sulphuric acid, these organisms represent the most extreme acidophiles known. So far, nothing is known about plasmid biology in these hyperacidophiles. Also, there are no genetic tools available for this genus. We have mobilized the 7.6 Kbp plasmid from P. oshimae in E. coli by introducing origin-containing transposons and described the plasmid in terms of its nucleotide sequence, copy