characterization of serum complement activity in serum of the komodo dragon (varanus komodoensis)描述血清血清补体活性的科莫多龙(varanus komodoensis).pdfVIP

Advances in Biological Chemistry, 2012, 2, 353-359 ABC /10.4236/abc.2012.24043 Published Online November 2012 (http://www.SciRP.org/journal/abc/) Characterization of serum complement activity in serum of the Komodo dragon ( Varanus komodoensis) 1* 1 1 2 3 Mark Merchant , Danyell Henry , Rodolfo Falconi , Becky Muscher , Judith Bryja 1Department of Chemistry, McNeese State University, Lake Charles, USA 2Department of Herpetology, San Antonio Zoo, San Antonio, USA 3Department of Herpetology, Houston Zoo, Houston, USA * Email: mmerchant@ Received 27 July 2012; revised 3 September 2012; accepted 13 September 2012 ABSTRACT three different mechanisms, the classical (antibody-de- pendent) pathway [1], the alternative pathway [2], and Incubation of different volumes of serum from the the lectin-dependent pathway [3]. Once activated, all Komodo dragon ( Varanus komodoensis) with sheep three mechanisms work in a proteolytic cascade fashion, red blood cells (SRBCs) resulted in volume-depend- resulting in the eventual formation of a multi-protein ent hemolysis, as measured spectrophotometrically at “membrane attack complex” [4] in the outer membrane 540 nm. The hemolysis occurred rapidly, with almost