conditional gene targeting dissecting the cellular mechanisms of retinal degenerations条件基因打靶解剖视网膜的细胞机制一种退化.pdfVIP

Hindawi Publishing Corporation Journal of Ophthalmology Volume 2011, Article ID 806783, 8 pages doi:10.1155/2011/806783 Review Article Conditional Gene Targeting: Dissecting the Cellular Mechanisms of Retinal Degenerations Yun-Zheng Le1, 2, 3, 4 1 Departments of Medicine, University of Oklahoma Health Sciences Center, 941 S. L. Young Boulevard, BSEB 302G, Oklahoma City, OK 73104, USA 2 Departments of Cell Biology, University of Oklahoma Health Sciences Center, 941 S. L. Young Boulevard, BSEB 302G, Oklahoma City, OK 73104, USA 3 Harold Hamm Oklahoma Diabetes Center, University of Oklahoma Health Sciences Center, 941 S. L. Young Boulevard, BSEB 302G, Oklahoma City, OK 73104, USA 4 Dean A. McGee Eye Institute, Oklahoma City, OK 73104, USA Correspondence should be addressed to Yun-Zheng Le, yun-le@ Received 2 July 2010; Accepted 11 November 2010 Academic Editor: Radha Ayyagari Copyright © 2011 Yun-Zheng Le. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Retinal neuron degeneration and survival are often regulated by the same trophic factors that are required for embryonic development and are usually expressed in multiple cell-types. Therefore, the conditional gene targeting approach is necessary to investigate the cell-specific function of widely expressed and developmentally regulated genes in retinal degeneration. The discussion in this review will be focused on the use of Cre/lox-based conditional gene targeting approach in mechanistic studies for retinal degeneration. In addition to the basic experimental designs, this article addresses various factors influe