cytotoxicity of seven recent dentine bonding agents on mouse 3t3 fibroblast cells细胞毒性七最近老鼠牙本质粘结剂3 t3成纤维细胞.pdfVIP

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cytotoxicity of seven recent dentine bonding agents on mouse 3t3 fibroblast cells细胞毒性七最近老鼠牙本质粘结剂3 t3成纤维细胞.pdf

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cytotoxicity of seven recent dentine bonding agents on mouse 3t3 fibroblast cells细胞毒性七最近老鼠牙本质粘结剂3 t3成纤维细胞

Open Journal of Stomatology, 2012, 2, 244-250 OJST /10.4236/ojst.2012.24043 Published Online December 2012 (http://www.SciRP.org/journal/ojst/) Cytotoxicity of seven recent dentine bonding agents on mouse 3T3 fibroblast cells Annette Olivier, Sias R. Grobler, Yusuf Osman Faculty of Dentistry, Oral and Dental Research Institute, University of the Western Cape, Cape Town, South Africa Email: srgrobler@uwc.ac.za Received 20 October 2012; revised 21 November 2012; accepted 30 November 2012 ABSTRACT step-etch and rinse and one step self-etch bonding agents were introduced. They gained rapid popularity because Today it is generally accepted that most bonding of their relative ease of use [4]. One step adhesives were agents are cytotoxic. In this study the relative cyto- made both acidic and hydrophilic to blend all adhesive toxicity of seven recent dentine bonding agents on components into one single solution. Available dental mouse 3T3 fibroblast cells were investigated. Materi- als and Methods. Near-confluent mouse 3T3 fibro- bonding agents which are used to bond resins to enamel and dentin differ in composition as well as proportions blast cells were exposed to Dulbecco Modified Eagle’s there-off. It is believed