detection of autoantibodies against recombinant desmoglein 1 and 3 molecules in patients with pemphigus vulgaris correlation with disease extent at the time of diagnosis and during follow-up检测重组desmoglein 1和3的自身抗体分子的寻常天疱疮患者与疾病相关性程度的诊断和随访中.pdfVIP

Hindawi Publishing Corporation Clinical and Developmental Immunology Volume 2009, Article ID 187864, 6 pages doi:10.1155/2009/187864 Clinical Study Detection of Autoantibodies against Recombinant Desmoglein 1 and 3 Molecules in Patients with Pemphigus vulgaris: Correlation with Disease Extent at the Time of Diagnosis and during Follow-Up Anna Belloni-Fortina,1 Diana Faggion,1 Barbara Pigozzi,2 Andrea Peserico,1 Matteo Bordignon,1 Vincenzo Baldo,3 and Mauro Alaibac1 1 Unit of Dermatology, University of Padua, Via Cesare Battisti 206, 35128 Padua, Italy 2 Veneto Institute of Oncology (IOV), IRCCS, Via Gattamelata 64, 35128 Padua, Italy 3 Department of Environmental Medicine and Public Health, Via Loredan 18, 35121 Padua, Italy Correspondence should be addressed to Mauro Alaibac, mauro.alaibac@unipd.it Received 14 April 2009; Revised 2 July 2009; Accepted 25 September 2009 Recommended by Ethan Shevach The recent availability of cDNA clones for pemphigus antigens has allowed the production of recombinant desmoglein 1 and desmoglein 3 molecules and the development of an ELISA approach in order to determine levels of antibodies to them. The aim of the study was to determine the relationship between autoantibodies levels and the extent of both mucosal and skin lesions in 20 patients with pemphigus vulgaris at the time of diagnosis and during follow-up. For the detection of autoantibodies by ELISA we used the recombinant proteins expressing overlapping sequences with the entire extracellular desmoglein 1 and desmoglein 3 domains. We showed that in presence of mucosal lesions there was a correlation between extension of mucosal involvement and autoantiboidies titres against both desmoglein 1 and desmoglein 3, whereas in presence of skin lesions there was a statistically signific