determination of magnesium in whole blood and serum of ischemic heart disease(ihd) patients by flame atomic absorption spectrometry测定全血和血清镁的缺血性心脏病(ihd)患者通过火焰原子吸收光谱法.pdfVIP

American Journal of Analytical Chemistry , 2011, 2, 996-1002 doi:10.4236/ajac.2011.28117 Published Online December 2011 (http://www.SciRP.org/journal/ajac) Determination of Magnesium in Whole Blood and Serum of Ischemic Heart Disease (IHD) Patients by Flame Atomic Absorption Spectrometry Hassan T. Abdulsahib Chemistry Department, College of Science, University of Basra, Basra, Iraq E-mail : lolaby2005@ Received October 10, 2011; revised November 15, 2011; accepted November 23, 2011 Abstract Flame atomic absorption spectrometric determination of magnesium in whole blood and serum of ischemic heart disease patients and control with different ages and sex was proposed. The limiting interfering phos- phate/magnesium ratio have been estimates. 2% w/v AlCl ·6H O was found to be very influential in remov- 3 2 ing phosphate interference effects. The detection limit was 0.065 μg/ml. Magnesium added to blood and se- rum sample, and carried through this method may be recovered completely (96% - 100%) recovery percent- age. The suggested method is simple, fast and selective. The statistical analysis of magnesium levels in blood and serum showed that blood and serum magnesium levels in patients were lower than magnesium contents of control group. Magnesium levels in blood and serum of males were significantly higher than females and the magnesium levels were age independent. These findings indicate that there was an association between blood and serum magnesium deficiency which can induce an entire array of path physiological phenomena known to be important in ischemic heart disease. Keywords: Magnesium, Whole Blood, Serum, Ischemic Disease, Atomic Absorption 1. Introduction