determination of mercuric ion in water samples with a led exciting and ccd based portable spectrofluorimeter测定水样中汞离子与一个令人兴奋的和基于ccd的便携式荧光光谱仪.pdfVIP

American Journal of Analytical Chemistry , 2011, 2, 605-611 doi:10.4236/ajac.2011.25068 Published Online September 2011 (http://www.SciRP.org/journal/ajac) Determination of Mercuric Ion in Water Samples with a LED Exciting and CCD Based Portable Spectrofluorimeter 1* 1 1 Arsenio Muñoz de la Peña , María Isabel Rodríguez-Cáceres , Diego Bohoyo Gil , María del Carmen Mahedero1 1 2 , María del Carmen Hurtado-Sánchez , Reyes Babiano 1 Department of Analytical Chemistry, University of Extremadura, Badajoz, Spain 2Department of Organic and Inorganic Chemistry , University of Extremadura, Badajoz, Spain * E-mail : arsenio@unex.es Received March 29, 2011; revised June 3, 2011; accepted June 15, 2011 Abstract The fluorescent characteristics of a fluorimetric chemosensor for mercuric ion, Hg2+, employing a synthe- sized Rhodamine 6G derivative, have been analyzed. For that, a portable spectrofluorimeter composed of a 515 nm LED as excitation source, two fiber-optics and a CCD camera as detector, has been used, intended for “in situ” analysis. A highly selective Rhodamine based probe for Hg2+, that is water soluble and gives a positive response upon analyte binding, is reported. The reagent is bearing a monothiospirolactone group in a Rhodamine 6G architecture and the thiol atom served for the direct attack of thiophilic Hg2+ . The fluores- cence enhancement is attributed to the spirolactone ring opening and the coordination of two sulphur atoms