determining the transcriptional regulation pattern of pgtip1 in transgenic arabidopsis thaliana by constructing gene确定pgtip1转录监管模式的构建转基因拟南芥的基因.pdfVIP

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determining the transcriptional regulation pattern of pgtip1 in transgenic arabidopsis thaliana by constructing gene确定pgtip1转录监管模式的构建转基因拟南芥的基因.pdf

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determining the transcriptional regulation pattern of pgtip1 in transgenic arabidopsis thaliana by constructing gene确定pgtip1转录监管模式的构建转基因拟南芥的基因

Advances in Bioscience and Biotechnology, 2010, 1, 384-390 ABB doi:10.4236/abb.2010.15051 Published Online December 2010 (http://www.SciRP.org/journal/abb/). Determining the transcriptional regulation pattern of PgTIP 1 in transgenic Arabidopsis thaliana by constructing gene coexpression networks* Haiying Chen1 1 1 2 1* , Lu Ying , Jing Jin , Qi Li , Weiming Cai 1Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Graduate School of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China; 2Genminix Informatics Co., Ltd. Email: wmcai@ Received 29 October 2010; revised 1 November 2010; accepted 1 November 2010. ABSTRACT Keywords: Arabidopsis thaliana ; Microarray; Coexpression Network; Transcription Factor Analysis; The seed size, seed mass, and growth rate of trans- PgTIP 1 genic Arabidopsis plants containing PgTIP 1, a gin- seng tonoplast aquaporin gene, are significantly 1. INTRODUCTION higher than those of wild-type Arabidopsis plants. Whole genome expression and bioinformatics analy- We screened differentially expressed genes using sup- sis, including analysis of co-expression networks and pression subtractive hybridization (SSH) between hor- transcription factors (Tfscan), were used to deter- mone-autotrophic and hormone-dependent ginseng cal- mine the key genes that are activated after the ex- lus lines and isolated and characterized an aquaporin pression of PgTIP 1 and the transcription