肺气虚证及肺阴虚证蛋白芯片探究.docVIP

肺气虚证及肺阴虚证蛋白芯片探究(1.安徽省中医院呼吸内科,安徽合肥 230031; 2.淮南市中医院呼吸内科,安徽淮南 232006; 3.北京华大吉比爱生物有限公司,北京101300) 摘 要:目的:探讨蛋白质芯片技术对肺气虚证和肺阴虚证的研究意义以及两证的血清IL-1β、IL-8、TNF-α表达水平。方法:将肺气虚证和肺阴虚证患者的血清通过Array- ELISA蛋白芯片平台检测。结果:COPD肺气虚组、COPD肺阴虚组、支气管哮喘肺气虚组、支气管哮喘肺阴虚组的血清IL-1β、IL-8、TNF-α水平明显高于健康组水平,差异有统计学意义(P0.05)。COPD肺气虚组、COPD肺阴虚组、支气管哮喘肺气虚组、支气管哮喘肺阴虚组点的灰度值(发光信号值)明显比健康组点的灰度值高;COPD肺阴虚组和支气管哮喘肺阴虚组点的灰度值比COPD肺气虚组和支气管哮喘肺气虚组的高;COPD肺气虚组点的灰度值与支气管哮喘肺气虚组的、COPD肺阴虚组点的灰度值与支气管哮喘肺阴虚组的相近。结论:蛋白质芯片技术用于肺气虚证和肺阴虚证是可行的;肺气虚和肺阴虚证是一种慢性以肺部为主的全身炎症综合症,且肺阴虚较肺气虚有加重趋势。 关键词:肺气虚证;肺阴虚证;蛋白质芯片? 中图分类号:R2-03 文献标识码:A 文章编号:1673-7717(2010)04-0705-03 Invastigation on Protein-microarray of Fei-Qi Deficiency Syndrome and Fei?Yin Deficiency Syndrome LI Ze?geng?1,WANG Guo?jun?2,PENG Bo?1,TONG Jia?bing?1, YANG Cheng?1,ZHANG Nian?zhi?1,JI Hong?yan?1,ZHANG Si?chun?1,ZHANG Wei?3 (1. Department of Respiratory Medicine, the Chinese Traditional Medicine Hospital of Anhui Province,Hefei 230031,Anhui,China; 2. Department of Respiratory Medicine, the Chinese Traditional Medicine Hospital of Huainan,Huainan 232006,Anhui,China; 3.Beijing BGI-GBI Biotech Co.Ltd.,Beijing 101300,China) [FQ(5。52,X-W] 收稿日期:2009-11-22 基金项目:国家自然科学基金资助项目 作者简介:李泽庚(1962-),男,安徽桐城人,主任医师,硕士,研究方向:中医药防治呼吸系统疾病。 通讯作者:王国俊(1974-),男,安徽舒城人,主治医师,硕士,研究方向:中医药防治呼吸系统疾病。 Abstract:Objective:Exploring the protein-microarray technology on Fei-Qi deficiency Syndrome and Fei-Yin deficiency Syndrome,and investigating the expression levels of IL-1β,IL-8,TNF-α in the serum.Methods:Detecting the serum of the patients of Fei-Qi deficiency Syndrome and Fei-Yin deficiency Syndrome through the Array-ELISA of protein-microarray platform.Results:The expression levels of IL-1β,IL-8,TNF-α in the serum of Fei-Qi deficiency Syndrome and Fei-Yin deficiency Syndrome in COPD group and Fei-Qi deficiency Syndrome and Fei-Yin deficiency Syndrome in bronchial asthma group are significantly higher than the levels of the healthy group(P Key words:Fei?Qi de