dna finger printing of prosopis cineraria and prosopis juliflora using issr and rapd techniquesdna指纹识别prosopis瓜叶菊和prosopis juliflora利用issr和rapd技术.pdfVIP
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dna finger printing of prosopis cineraria and prosopis juliflora using issr and rapd techniquesdna指纹识别prosopis瓜叶菊和prosopis juliflora利用issr和rapd技术
American Journal of Plant Sciences , 2011, 2, 527-534 527
doi:10.4236/ajps.2011.24062 Published Online October 2011 (http://www.SciRP.org/journal/ajps)
DNA Finger Printing of Prosopis cineraria and
Prosopis juliflora Using ISSR and RAPD
Techniques
Khaled Elmeer, Ameena Almalki
Genetic Engineering Department, Biotechnology Center, Doha, Qatar.
Email: elmeer@
th th th
Received July 17 , 2011; revised August 16 , 2011; accepted September 5 , 2011.
ABSTRACT
Diversity within and among the populations of Prosopis cineraria and Prosopis juliflora collected from different loca-
tion of Qatar were explored using Inter Simple Sequence Repeat (ISSR) and Random Amplified Polymorphic DNA
(RAPD) markers. A total of one hundred and nine bands were generated from twenty nine ISSR and nineteen bands
from seven RAPD primers with an average polymorphism of more than ninety nine percent across all the genotypes.
ISSR techniques were capable of distinguishing between P. cineraria and P. juliflora, through twenty one bands. How-
ever, of the seven RAPD markers, only three bands were able to distinguish between Prosopis species. The dendro-
grams for the analysis of genetic similarity show that the individuals from both species can be separated in two highly
related groups. Our observations suggest that genetic variations among different accessions of Prosopis are identified
using ISSR and RAPD analysis.
Keywords : Ghaf, Ghawiaf, Polymorphism, Dendrogram and Genetic Diversity
1. Introduction The accelerated and uncontrolled harvesting of this
important natural resource has led to land degradat
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