effect of cyclosporin-a on the blood–retinal barrier permeability in streptozotocin-induced diabetes非对血视网膜屏障的影响渗透在体外糖尿病.pdfVIP

Research Paper Mediators of Inflammation, 9, 243 –248 (2000) Background: Our previous results showed that in Effect of cyclosporin-A on the retinas from streptozotocin (STZ)-induced diabetic rats there is an increased level of interleukin-1b (IL- blood– retinal barrier permeability in 1b ). This cytokine may be involved in the expression streptozotocin-induced diabetes of the inducible isoform of the nitric oxide synthase (iNOS), with consequent synthesis of large amounts of NO and bloodÐ retinal barrier (BRB) breakdown. Aims: The aim of this work was to examine whether Analia´ Carmo,1 Jose´ G. Cunha-Vaz,1 the administration of cyclosporin-A (Cs-A) to STZ- Arselio´ P. Carvalho2 and Maria Celeste Lopes2 ,C A induced diabetic rats inhibits the synthesis of IL-1b and the expression of the inducible proteins, iNOS and cyclo-oxygenase-2 (COX-2) in retinal cells, and whether the activity of these proteins contribute to 1Center of Ophthalmology, Institute for Biomedical BRB breakdown. Research on Light and Image, University of Methods: The level of IL-1b was evaluated by ELISA Coimbra, Coimbra, Portugal; 2 Center for 3 and the NO production by L-{ H}-citrulline formation. Neurosciences of Coimbra, Department of Zoology, Expression of iNOS and COX-2 proteins was deter- University of Coimbra, Coimbra, Portugal mined by two methods, western blot and immunohis- tochemistry. The permeability of the BRB was assessed by quantification of the vitreous protein. Results and discussion: Our results indicated that the CA Corresponding Author levels of IL-1b and N