菠萝蛋白酶软膏对豚鼠三度烧伤酶溶痂作用观察.docVIP

菠萝蛋白酶软膏对豚鼠三度烧伤酶溶痂作用观察摘要：目的观察菠萝蛋白酶软膏用于豚鼠三度实验性烧伤的酶溶痂作用。方法用豚鼠造成三度烧伤模型，以湿润烧伤膏为阳性对照，菠萝蛋白酶软膏基质为阴性对照，观察菠萝蛋白酶软膏用于豚鼠三度烧伤的平均脱痂时间，并对脱痂组织进行病理学检查。结果菠萝蛋白酶软膏用于豚鼠三度烧伤的平均脱痂时间同阳性对照组，明显少于阴性对照组（P＜0.01）。光镜下显示菠萝蛋白酶软膏组的病理结果与阳性对照组相同。结论菠萝蛋白酶软膏用于豚鼠三度烧伤具有良好的酶溶痂作用。 关键词：菠萝蛋白酶软膏；三度烧伤；酶溶痂 中图分类号：R644文献标识码：A文章编号：1672-979X(2008)11-0027-03 Observation of Enzymatic Debridement Effect of Bromelain Ointment on Guinea Pigs with Third-Degree Burns SU Huai1, LI Nai-cheng1, HE Yan-li1, ZHANG Tian-min2 (1. Shandong Bausch Lomb Freda Phar. Co., Ltd., Jinan 250014, China; 2. School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China) Abstract：Objective To investigate the enzymatic debridement effect of bromelain ointment on guinea pigs with third-degree burns. Methods The time for debridement of bromelain ointment on guinea pigs with third-degree burns was recorded with ointment base as negative control and moist exposed burn ointment as positive control. The pathological examination of tissue suffering from enzymatic debridement was also conducted. Results The average time for debridement of bromelain ointment on guinea pigs with third-degree burns was consistent with that of moist exposed burn ointment. Both of them were more effective than negative control（P＜0.01）. The pathological sections showed that there was no difference in tissue morphology for bromelain ointment and moist exposed burn ointment. Conclusion Bromelain ointment has a satisfactory enzymatic debridement effect on guinea pigs with third-degree burns. Key words：bromelain ointment; third-degree burns; enzymatic debridement 菠萝蛋白酶（bromelain）是从凤梨属菠萝中提取的一组巯基蛋白水解酶，商品化菠萝蛋白酶来源于菠萝茎中。此酶对三度烧伤动物模型具有良好的酶溶痂作用，可去除坏死组织，对周围正常组织无影响，无全身毒性。此酶的制剂已在日本等国开发成药品，用于烧伤、褥疮、浅表性溃疡、多种外伤及手术后坏死组织的分解、去除等，目前国内尚无相关产品。我们通过豚鼠三度烧伤模型，观察了自制菠萝蛋白酶软膏的酶溶痂作用。 1材料与仪器 1.1材料 短毛豚鼠40只，普通级，纯白色，由北京维通利华实验动物技术有限公司提供，许可证号：SCXK（京）2007-0001，体重290～350 g，雌雄兼用。 菠萝蛋白酶软膏（自制，批号070801，63 000酪蛋白单位/g）；软膏基质：按软膏处方配制，不加药物；湿润烧伤膏（批汕头市美宝制药公司）；硫化钠脱毛剂（硫