effective procedure for development of est-ssr markers using cdna library有效利用cdna est-ssr标记库的发展过程.pdfVIP

下载本文档

0
0
约3.95万字
约 6页
2017-08-28 发布于上海
举报
版权申诉

effective procedure for development of est-ssr markers using cdna library有效利用cdna est-ssr标记库的发展过程.pdf

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

effective procedure for development of est-ssr markers using cdna library有效利用cdna est-ssr标记库的发展过程

American Journal of Plant Sciences, 2012, 3, 1322-1327 /10.4236/ajps.2012.39159 Published Online September 2012 (http://www.SciRP.org/journal/ajps) Effective Procedure for Development of EST-SSR Markers Using cDNA Library 1 1 2 2* Kyung A Kim , Hee-Cheon Park , Jae-Keun Sohn , Kyung-Min Kim 1Department of Biology, College of Natural Sciences, Kyungpook National University, Daegu, Korea (South); 2School of Applied Biosciences, College of Agriculture and Life Science, Kyungpook National University, Daegu, Korea (South). Email: Kyung-AKim, hikyunga@knu.ac.kr, Hee-Cheon Park, heecheon@knu.ac.kr, Jae-Keun Sohn, jhsohn@knu.ac.kr, * Kyung-Min Kim, kkm@knu.ac.kr Received August 8th, 2012; revised September 5th, 2012; accepted September 13th, 2012 ABSTRACT The present study was conducted to develop EST-SSR markers using the cDNA library from rice plant. Total RNA ex- tracted from the leaves of brown plant hopper resistance gene originated from a rice cultivar “Cheongcheong” and sen- sitive rice cultivar “Nakdong” were used to synthesize a cDNA library. As a result of analyzing the cDNA library, the 17 EST-SSR primer sets were developed. This study enables to provide effective marker assisted selection (MAS) methods on the selection of white-backed planthopper resistance gene originated from a rice plant more simply, quickly and precisely. Furthermore, using this marker’s advantage of deriving from cDNA, it is possible to identify the white-backed planthopper resistance gene. In addition, this study introduces a technique for construction of a cDNA library safely without using radioactivity. Keywords: cDNA; EST-SSR Markers; Radioactivity; Rice 1. Introduction 2. Materials and Methods Several protocols for cDNA library construction