effectiveness of gelatin solidification for unfrozen preservation and transportation of astrocytes有效性的凝胶固化解冻星形胶质细胞的保存和运输.pdfVIP

Advances in Biological Chemistry, 2012, 2, 238-242 ABC /10.4236/abc.2012.23029 Published Online August 2012 (http://www.SciRP.org/journal/abc/) Effectiveness of gelatin solidification for unfrozen preservation and transportation of astrocytes 1 1 2 1* Nobuko Ishibashi , Shinichi Sotome , Kazuyuki Nakajima , Akio Shimizu 1Department of Environmental Engineering for Symbiosis, Faculty of Engineering, Soka University, Tokyo, Japan 2Department of Bioinformatics, Faculty of Engineering, Soka University, Tokyo, Japan * Email: shimizu@soka.ac.jp Received 17 June 2012; revised 19 July 2012; accepted 24 July 2012 ABSTRACT attempted to use gelatin, which solidifies at about 15˚C - 20˚C. Gelatin is known to be safe for cells, as gelatin- We herein studied a non-cryogenic cell preservation coated dishes are routinely used for cell culture, and and transportation method. We found that astrocytes gelatin was used as a solid medium before agar [13,14]. can be preserved in an unfrozen state at 4˚C (without For this experiment, we selected astrocytes, which are medium exchange), while maintaining good condition a sub-type of the central nervous glial cells, and are the in comparison with cryopreservation for the periods