effectiveness of selected reaction monitoring for rapid assay of cypermethrin residue in perilla leaves选择反应监测的有效性的快速测定氯氰菊酯残留在紫苏属树叶.pdfVIP

Advances in Materials Physics and Chemistry , 2011, 1, 1-5 doi:10.4236/ampc.2011.11001 Published Online June 2011 (http://www.SciRP.org/journal/ampc) Effectiveness of Selected Reaction Monitoring for rapid assay of Cypermethrin Residue in Perilla Leaves Noriyasu Niimura DATUM Solution Business Operations, JEOL Ltd., Tokyo, Japan E-mail : niimura@jeol.co.jp Received May 20, 2011; revised June 14, 2011; accepted June 25, 2011 Abstract Many kinds of pesticides have been developed and used to yield a good harvest but the residues in agricul- tural products cause health problems. It is important to keep watch on these residues by using adequate methods of analysis. Pretreatment such as gel permeation chromatography (GPC) or column chromatography is often needed for the quantitative analysis of pesticide in agricultural products by conventional methods such as gas chromatography/low resolving power mass spectrometry (GC/LRMS). However, these pretreat- ments need a lot of work and take time. New methods saving the necessity of these pretreatments have been desired. We have applied selected reaction monitoring (SRM) to quantitatively determine cypermethrin residues in Perilla frutescens samples and compared the results with LRMS and HRMS in SIM mode. A background peak caused by the matrix overlapped the cypermethrin peak in the analysis using LRMS. SRM and HRMS in SIM mode provided chromatograms without matrix interference. The high selectivity of the product ion (m/z 127) produced from precursor ion (m/z 163) isolated the target peaks from the matrix peaks when using SRM. This method eliminates the pretreatment step, thus saving time and simplify ing the ana- lytical process. Keywords: Pesticide Residue,