蓝桉叶挥发油化学成分气相色谱-质谱研究探究.docVIP

蓝桉叶挥发油化学成分气相色谱-质谱研究探究摘要：目的研究云南产蓝桉叶挥发油的化学成分。方法采用水蒸气蒸馏法提取云南产蓝桉叶挥发油，经气相-质谱联用仪分析其成分，采用峰面积归一化法计算各组分的相对含量。结果确定了云南产蓝桉叶挥发油中47个化合物，所鉴定成分占总馏出峰面积的99.17 %，其主要成分是1,8-桉叶素（72.71 %），α-蒎烯（9.22 %），α-松油醇醋酸酯（3.11 %），(-)-蓝桉醇（2.77 %），α-松油醇（2.54 %），别香橙烯（2.47 %）。结论确定了云南产蓝桉叶挥发油的化学成分，为综合利用云南产蓝桉叶挥发油奠定了基础。 关键词：云南蓝桉叶；挥发油；化学成分；气相色谱-质谱 中图分类号：R914文章标识码：A文章编号：1672-979X(2009)01-0030-03 Study on GC-MS Analysis of Volatile Oil Constituents from Eucalyptus globulus Labill. Leaf SONG Ai-hua1, WANG Ying2, LIU Yan-mei1 (1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China; 2. Tianjin Key Laboratory of Chemistry and Analysis of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China) Abstract：Objective To study the volatile oil constituents from Eucalyptus globulus Labill. leaf collected in Yunnan Province of China. Methods The volatile oil constituents were extracted by steam distillation from Eucalyptus globulus Labill. leaf, and analyzed by GC-MS. The relative amount of each constituent was calculated by peak area normalization method. Results Forty-seven volatile oil constituents from Eucalyptus globulus Labill. leaf were identified which covered 99.17 % of total GC peak areas. The main compounds were 1,8-eucalyptol(72.71 %), α-pinene(9.22 %), α-terpineol acetate(3.11 %), (-)-globulol(2.77 %), α-terpineol(2.54 %), alloaromadendrene (2.47 %). Conclusion The volatile oil constituents from Eucalyptus globulus Labill. leaf in Yunnan Province can be successfully determined and identified, which provides the foundation for the further comprehensive utilization of volatile oils. Key words：Eucalyptus globulus Labill. leaf; volatile oil; chemical constituent; gas chromatography-mass spectrometry (GC-MS) 蓝桉（Eucalyptus globulus Labill.）别名玉树、杨草果树、灰杨柳等，为桃金娘科Myrtaceae植物，以叶和果实入药。原产澳大利亚，现广泛分布于我国云南、福建、广东、广西、四川等省区。蓝桉叶和幼枝含挥发油，经水蒸气蒸馏即可得桉油。桉叶含挥发油、苦味质、鞣质和树脂等多种成分，挥发油占3 %～6 %，其中含有的多数成分为萜类、萜醇类、醚、酮和酯等化合物[1]，具有疏风解热、祛湿解毒、杀虫等功能，已由中国药典收载[