effects of plasma proteins on staphylococcus epidermidis rp62a adhesion and interaction with platelets on polyurethane biomaterial surfaces血浆蛋白对葡萄球菌epidermidis rp62a与聚氨酯生物材料表面血小板粘附和交互.pdfVIP

Journal of Biomaterials and Nanobiotechnology, 2012, 3, 487-498 487 /10.4236/jbnb.2012.324050 Published Online October 2012 (http://www.SciRP.org/journal/jbnb) Effects of Plasma Proteins on Staphylococcus epidermidis RP62A Adhesion and Interaction with Platelets on Polyurethane Biomaterial Surfaces Li-Chong Xu1*, Christopher A. Siedlecki1,2 1Department of Surgery, Biomedical Engineering Institute, College of Medicine, The Pennsylvania State University, Hershey, USA; 2Department of Bioengineering, Biomedical Engineering Institute, College of Medicine, The Pennsylvania State University, Hershey, USA. * Email: lxx5@ Received August 11th, 2012; revised September 13th, 2012; accepted September 24th, 2012 ABSTRACT Plasma proteins influence the initial adhesion of bacteria to biomaterials as well as interactions between bacteria and blood platelets on blood-contacting medical devices. In this paper, we study the effects of three human plasma proteins, albumin, fibrinogen (Fg), and fibronectin (Fn), on the adhesion of Staphylococcus epidemidis RP62A to polyurethane biomaterial surfaces, and also address how these three proteins affect bacterial interactions with human platelets on ma- terials. Measurements of bacterial adhesion on polymer surfaces pre-adsorbed with a variety of proteins demonstrate that Fn leads to increased bacterial adhesion, with the order of effectiveness being Fn  Fg albumin. Immuno-AFM (atomic force microscopy) was used to assess the Fn adsorption/activity on surfaces and bacterial cell membranes by looking at molecular scale events. A correlation between molecular scale Fn adsorption and macroscale bacterial adhe- sion was observed, with an increased numbers of Fn-receptor recognition events measured on cell surfaces as compared to Fg-receptor recognition events, suggesting Fn is an important pro