原创力文档effects of preparation and storage of agar media on the sensitivity of bacterial forward scattering patterns琼脂的制备和储存媒体的影响的敏感性细菌向前散射模式.pdfVIP
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effects of preparation and storage of agar media on the sensitivity of bacterial forward scattering patterns琼脂的制备和储存媒体的影响的敏感性细菌向前散射模式
Open Journal of Applied Biosensor, 2012, 1, 26-35
/ 10.4236/ojab.2012.13004 Published Online November 2012 (http://www.SciRP.org/journal/ojab)
Effects of Preparation and Storage of Agar Media on the
Sensitivity of Bacterial Forward Scattering Patterns
1 2 2 3 2*
Mélissa Mialon , Yanjie Tang , Atul K. Singh , Euiwon Bae , Arun K. Bhunia
1Institut National Supérieur, Agrosup, Dijon, France
2Department of Food Science, Purdue University, West Lafayette, USA
3School of Mechanical Engineering, Purdue University, West Lafayette, USA
*
Email: bhunia@
Received September 14, 2012; revised October 19, 2012; accepted October 27, 2012
ABSTRACT
Recent worldwide foodborne outbreaks emphasize the need for the development of rapid and accurate method for
pathogen detection. To address such issues, a new colony based label-free detection method working on the principles
of elastic light scattering was introduced. In order to build libraries of scattering images for bacterial pathogens, it is
pertinent to determine the effect of preparation and storage of the agar media on the scatter patterns. Scatter patterns of
three Escherichia coli serovars (O26, O111 and O157) were studied and used in a model system, after growth on Sorbi-
tol-MacConkey agar plates that were prepared and stored at different conditions in the laboratory. Quantitative image
processing software was used to analyze variation in scatter patterns of the same serovar on media prepared under vari-
ous standard laboratory conditions and to generate a cross-validati
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