effects of sea salts on induction of cell proliferation in liquid cultures of mangrove plants, sonneratia caseolaris and s. alba海上盐对诱导细胞增殖的影响在液体培养基的红树林植物,sonneratia caseolaris和阿尔巴.pdfVIP

American Journal of Plant Sciences , 2011, 2, 35-42 35 doi:10.4236/ajps.2011.21004 Published Online March 2011 (http://www.SciRP.org/journal/ajps) Effects of Sea Salts on Induction of Cell Proliferation in Liquid Cultures of Mangrove Plants, Sonneratia caseolaris and S. alba 1 1 2 2 Raiki Yamamoto , Yoshifumi Kawana , Reiko Minagawa , Hamako Sasamoto 1Graduate School of Environment and Information Sciences, Yokohama National University, Yokohama, Japan; 2Faculty of Envi- ronment and Information Sciences, Yokohama National University, Yokohama, Japan. Email: sasamoto@ynu.ac.jp Received December 1st, 2010; revised December 13th, 2010; accepted December 29th, 2010. ABSTRACT The effects of five salt ingredients of sea water, KCl, NaCl, CaCl , MgCl and MgSO , on induction of cell proliferation 2 2 4 in Sonneratia caseolaris were investigated. Proliferation was examined in tissue explants derived from such as leaves, cotyledons, and hypocotyls using a small-scale liquid culture method. Addition of 12.5-25 mM of MgCl2 was unique in stimulating cell proliferation in all tissues of S. caseolaris. Otherwise, different effects of salts were observed among the three tissues. In hypocotyl culture, 25-50 mM of NaCl and CaCl2 stimulated cell divisions. Tolerance to 100 mM of MgSO4 was observed in leaves. Three osmotically active compounds commonly used in tissue culture, sorbitol, manni- tol and glycinebetaine, were also tested to assess the importance of osmotic effects on cell proliferation. No significant stimulation by these was observed over a wide range of concentrations. Data were compared with those of cotyledon cultures of another mangrove, S. alba, which