effect of vitamins on in vitro organogenesis of plant维生素对体外器官形成的植物.pdfVIP

American Journal of Plant Sciences , 2011, 2, 669-674 669 doi:10.4236/ajps.2011.25080 Published Online November 2011 (http://www.SciRP.org/journal/ajps) Effect of Vitamins on In Vitro Organogenesis of Plant * Peter Abrahamian, Arumugam Kantharajah Department of Agricultural Sciences, American University of Beirut, Riad El Solh, Beirut, Lebanon. Email: a.kantharajah@ th th st Received June 10 , 2011; revised July 25 , 2011; accepted September 1 , 2011. ABSTRACT Vitamins are necessary compounds synthesized and utilized in plants. In tissue culture media, vitamin addition is not always common; since the amount needed by plants is relatively unknown and varies. Vitamins, in combination with other media constituents, have been shown to have direct and indirect effects on callus growth, somatic growth, rooting, and embryonic development. For example, different studies have shown that thiamine is associated with cytokinin and has a role in inducing callus growth and rooting. Moreover, thiamine was essential in facilitating the production of more secondary metabolites such as proteases in pineapple. Both biotin and riboflavin play a role in callus develop- ment as well. Specifically, riboflavin exerts different effects on plant rooting either positively and negatively. Vitamin D known to cause uptake of calcium in animal tissue, exerts a similar effect in plants. In addition, vitamin D causes cell elongation and meristematic cell division. Vitamin C, known for its anti-oxidative properties, has also enhanced shoot growth and rooting. Keywords : Vitamin, Organogenesis, In Vitro, Plant Tissue Culture, Plant Propagation 1. Introduction lase [4]. Pyruvate decar