原创力文档effect of vitrification procedure on chromosomal status of embryos achieved from vitrified and fresh oocytes玻璃化过程对染色体的影响胚胎的状态实现从陶瓷和新鲜的卵母细胞.pdfVIP
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effect of vitrification procedure on chromosomal status of embryos achieved from vitrified and fresh oocytes玻璃化过程对染色体的影响胚胎的状态实现从陶瓷和新鲜的卵母细胞
Vol.3, No.7, 467-476 (2011) Health
doi:10.4236/health.2011.37077
Effect of vitrification procedure on chromosomal status
of embryos achieved from vitrified and fresh oocytes
*
Javier Israel García , Luis Noriega-Portella, Luis Noriega-Hoces
Laboratory of Assisted Reproduction, PRANOR Group of Assisted Reproduction, Concebir Clinic, Lima, Peru.
*
Corresponding Author: jgarciaf@
Received 27 April 2011; revised 9 June 2011; accepted 28 June 2011.
ABSTRACT Keywords: Oocytes; Vitrification; PGD; FISH; ART
background: In order to assess the chromoso- 1. INTRODUCTION
mal status in embryos obtained from vitrified
and fresh donated oocytes, preimplantational Human oocytes cryopreservation is an attractive
genetic diagnostic (PGD) was performed after choice to the range of fertility treatments presently of-
biopsy of one blastomere at day 3. METHODS: A fered. Since the very first frozen oocyte pregnancy was
total of 249 oocytes were obtained from 23 oo- achieved in humans by Chen [1], the slow cooling me-
cyte donors, 80 oocytes were used in the vitri- thod has been applied with varying success [2-7]. There
fied group and 151 oocytes were used in the are many reports about the cryopreservation of human
fresh group. Nine chromosomes (13, 15, 16, 17, oocytes with good fertilization and cleavage rates [1,8]
18, 21, 22, X and Y) were investigated by fluo- but with variable results on pregnancy rates per cycle
rescence in situ hybridization
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