effects of subminimum inhibitory concentrations of antibiotics on the pasteurella multocida proteome a systems approachsubminimum抑制浓度的抗生素对巴斯德菌multocida蛋白质组的系统方法.pdfVIP

Hindawi Publishing Corporation Comparative and Functional Genomics Volume 2008, Article ID 254836, 12 pages doi:10.1155/2008/254836 Research Article Effects of Subminimum Inhibitory Concentrations of Antibiotics on the Pasteurella multocida Proteome: A Systems Approach Bindu Nanduri,1, 2 Mark L. Lawrence,1, 2 Divya Swetha Peddinti,1, 2 and Shane C. Burgess1, 2, 3, 4 1 College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA 2 Mississippi State University Institute for Digital Biology, Mississippi State University, Mississippi State, MS 39762, USA 3 Mississippi Agriculture and Forestry Experiment Station, Mississippi State University, Mississippi State, MS 39762, USA 4 MSU Life Sciences and Biotechnology Institute, Mississippi State University, Mississippi State, MS 39762, USA Correspondence should be addressed to Bindu Nanduri, bnanduri@cvm.msstate.edu Received 6 December 2007; Accepted 19 February 2008 Recommended by John Parkinson To identify key regulators of subminimum inhibitory concentration (sub-MIC) antibiotic response in the Pasteurella multocida proteome, we applied systems approaches. Using 2D-LC-ESI-MS2 , we achieved 53% proteome coverage. To study the differential protein expression in response to sub-MIC antibiotics in the context of protein interaction networks, we inferred P. multocida Pm70 protein interaction network from orthologous proteins. We then overlaid the differential protein expression data onto the P. multocida protein interaction network to study the bacterial response. We identified proteins that could enhance antimicrobial activity. Overall compensatory response to antibiotics was characterized by altered expression of proteins involved in purine metabolism, stress response, and cell envelope permeabili