黄芩苷对人黑色素瘤A375细胞增殖影响.docVIP

黄芩苷对人黑色素瘤A375细胞增殖影响摘 要：目的：探讨黄芩苷对人黑色素瘤A375细胞株体外增殖的抑制作用。方法：选用人黑色素瘤A375细胞进行体外培养，以不同浓度的黄芩苷分别处理黑色素瘤A375细胞，连续6天使用倒置显微镜进行观察，计数，绘制生长曲线；以不同浓度(0．1～50μmol／L)的黄芩苷分别处理黑色素瘤A375细胞细胞24、48、72h后，用MTT法检测细胞生长抑制率；流式细胞仪检测细胞凋亡率。结果：生长曲线及MTT法显示黄芩苷对黑色素瘤A375细胞有抑制作用，并呈剂量和时间依赖性；流式细胞仪检测到细胞在黄芩苷作用后，细胞凋亡率增加。结论：黄芩苷对人黑色素瘤A375细胞具有明显抑制作用，细胞凋亡可能是其机制之一。 关键词：黄芩苷；黑色素瘤A375细胞；MTT 中图分类号：R285.6 文献标识码：A文章编号：1673-7717（2008）11-2510-03 Inhibitory Effect of Baicalim on Human Melanoma A375 Cell Line KUANG Ju?xiang?1，LEI Pei?hong?2，SHI Jiang?1 （1.The Affiliated Hospital of Zunyi Medical College，Zunyi 563003，Guizhou,China； 2.Orthopaedics Hospital of Zunyi,Zunyi 563003,Guizhou,China） Abstract：Objective：To investigate the inhibitory effect of Baicalim on human Melanoma A375 cell line in vitro．Methods:Melanoma A375 cells were cultured invitro and then treated with different concentrations(0.1~50μmol/L) of Baicalim solution.Cells were observed with inverted microscope，the growing curve was drawn．MTT assay was adopted to determine the inhibition rate of Baicalim on melanoma A375 cells；Flow cytometry was used to detect the apoptosis of melanoma A375 cells．Results:The growing curves and MTT assay showed that different concentrations of Baicalim were cytotoxic to melanoma A375 cells in a time-dependent and dose-dependent manner．The FCM analysis showed that the apoptosis rate of melanoma A375 cells was increased after treatment of Baicalim.Meianoma A375 cells presented the characteristic morphological changes of apoptosis under inverted microscope.Conclusion：Baicalim could inhibit the proliferation of human melanoma A375 cells invitro，which way associated with cell apoptosis. Key words：Baicalim；human melanoma A375 cell line;MTT 黑色素瘤是最常见的皮肤恶性肿瘤，转移早，严重威胁着人类的健康和生命，目前临床尚无根治的有效药物，因此急需寻找有效的治疗药物。中药抗肿瘤的作用日益引起人们的关注，黄芩苷(baicaiim C21H18O11)为黄芩-T-葡萄糖醛酸苷，是从中药黄芩(Scutcllaria Baicalensis Georgi)中提取的。黄芩是我国重要的常用传统中药，是唇形科植物黄芩的干燥根。最早收载于《神农本草经》，列为草根药的上品。它主要含黄酮类化合物，具有广泛药理作用的活性物质，现代药理研究表明黄芩具有抗氧化，抗病毒、抗真菌，抗过敏，抗炎，抗癌等方面的药理作