CTGF+siRNA质粒对UUO小鼠肾脏间质纤维化影响.pdfVIP

中 文 摘 要 有潜力成为防治肾脏间质纤维化的一种新策略。 关键词：结缔组织生长因子；小分子干扰 RNA ；肾纤维化；转化生 长因子β1；α-平滑肌肌动蛋白 3 英 文 摘 要 Effect of CTGF siRNA plasmid on renal interstitial fibrosis in UUO mice ABSTRACT Objective: Renal interstitial fibrosis is a common pathway of the development of a variety of kidney disease to end-stage renal disease. Renal interstitial myofibroblasts is the important cells to produce interstitial extracellular matrix (extracellularmatrix, ECM). Transforming growth factor β1 (TGF-β1) is a recognized factor-induced fibrosis. It can activate the mechanisms of renal tubular epithelial cells to tubular epithelial - myofibroblast ransdifferentiation, and thus expression of α-smooth muscle actin (α-SMA), Synthesis Ⅰ, Ⅳ collagen (collagen Ⅰ and IV), fibronectin and other extracellular matrix components involved in the process of renal interstitial fibrosis. Alpha-smooth muscle actin (α-SMA) is a specific marker of the muscle fibroblast, and is a cytoskeletal protein. In addition to vascular smooth muscle in normal renal tissue, few expressed α-SMA. But the renal interstitial cell cytoplasm, the basal side of renal tubular epithelial cells, and some atrophic epithelial cells of the small tubes can express a-SMA when the renal occured interstitial fibrosis. Collagen Ⅰ is the main collagen, widespread in a variety of connective tissue. It plays an indispensable role in renal fibrosis development. Its over-expression is an important part of the formation of renal interstitial fibrosis. Connective tissue growth factor (CTGF) is a profibrotic