气相色谱法分析不同产地辣木叶中植物甾醇的含量-食品安全质量检测.PDF

7 8 Vol. 7 No. 8 2016 8 Journal of Food Safety and Quality Aug. , 2016 * 杨 扬, 赵志红 , 李小佳, 朱 慧 (, , 310018) 摘 要: 目的 β 方法 50%, :(1:1, V:V), N,N- ::(8:2:1, V:V:V), , β 结果 β 20 mg/100 g , 40 mg/100 g,30 mg/100 g ; 5~7 mg/100 g, 8 mg/100 g, 10 mg/100 g;2~12 mg/100 g 结论 β , 关键词: ; ; Content determination of phytosterol in Moringa oleifera leaves from different areas by gas chromatography * YANG Yang, ZHAO Zhi-Hong , LI Xiao-Jia, ZHU Hui (Key Laboratory of Food and Biological Engineering of Zhejiang Province, Hangzhou Wahaha Group Co., Ltd ., Hangzhou 310018, China) ABSTRACT: Objective To study the content difference of campesterol, β-sitosterin, and stigmasterol in Moringa oleifera leaves from Xishuangbanna, Shaoguan, Angola, India and Yunnan. Methods After saponification by 50% potassium hydroxide, the free phytosterol of samples were extracted by diethyl ether:hexane(1:1, V:V), and the solvent were evaporated to dryness before silanization using N,N-Dimethylformamide(DMF), hexamethyldisilazane (HMDS) and trimethyl-chlorosilane (TMCS)(8:2:1, V:V:V). The content