1型糖尿病骨质疏松大鼠模型的建立及评价.pdfVIP

中国骨质疏松杂志 2016 年 5 月第 22 卷第 5 期 Chin J Osteoporos , May 2016 ,Vo122 , No. 5 532 Published online www. wanfangdate. com. cn doi: 10. 3969/j. issn. 1006-710唱 2016. 05.004 --t仓辛苦· 1 型糖尿病骨质疏松大鼠模型的建立及评价 张百缓1 • 纳青青l 吴天秀1 周满如2 李近2 刘银输2 1.广东医学院解剖学教研室，湛江 524023 2. 广东医学院药理学教研室，湛江 524023 中图分类号: R587. 1 文献标识码: A 文章编号: 1006-7108 (2016) 05-0532 -04 摘要:目的 建立 I 型糖尿病性骨质疏松大鼠模型，通过骨形态计量学观察该模型的改变。方法 选取 3 月龄雄性 5D 大鼠 60 只，随机分为正常对照组(30 只)和造模组(30 只) ，造模组腹腔注射 5TZ60 mg/kg ，诱导建立 I 型糖尿病性骨质疏松大鼠模 型。结果 从血糖、体重、骨密度、骨组织形态计量学证实该方法可以成功制备 l 型糖尿病性大鼠模型。结论 该方法制备的 I 型糖尿病性骨质疏松大鼠模型，成模率高，模型稳定。 关键询: 1 型糖尿病模型;糖尿病性骨质疏松;骨形态计量学 Establishment and evaluation of rat type 1 diabetes and osteoporosis model ZHANG Liyuan , NA Qingqing , WU Tianxiu , ZHOU Manru2 , LI Jin2 , LIU Yuyu2 1. Department of Anatomy 2. Department of Pharmacy , Guangdong Medical College , Zhanjiang 524023. China Corresponding author: ZHANG Liyuan , Email: zmdjzly@126.com Abstract: Objective To establish type 1 diabetic osteoporosis rat model , and to observe the change of the model using bone histomorphometry. Methods Sixty 3-month-old male Sprague-Dawley (SD) rats were randomly divided into normal control group (30 rats) and diabetic model group (30 rats). Rats in the model group received intraperitoneal STZ i时ection (60 mg/kg) to establish type 1 diabetic osteoporosis rat mode l. Results The success of type 1 diabetic osteoporosis rat model was confirmed by determination of blood glucose , body weight. bone mineral density , and bone tissue morphology. Conclusion This method to develop type 1 diabetic osteoporosis rat model is efficient and stable. Key words: Type 1 diabetic model; Diabetic osteoporosis; Bone histomorphometry