4个多用途萱草品种离体快繁体系的建立.pdfVIP

安徽农业科学，Journal of Anhui A伊. Sci. 2014 ,42( 14) :4189 -4191 ,4214 责任编辑石金友责任校对李岩 4 个多用途董草晶种离体快繁体系的建立 储博彦1 气张全锋1 气高超利3 ，李金霞1 ， 2 ，赵玉芬1 ， 2 (i河北省林业科学研究院，河北石家庄 05∞61 ;2 河北省林木良种工 程技术研究中心，河北石家庄 05∞61 ;3. 河北农业大学园林与旅游学院，河北保定 071∞0) 摘要 [目的]建立4 个多用途莹苹品种的离体快繁体系。[方法]以4 个多用途莹苹品种为试材，研究消毒时间对不同外植体的影响， 以及不同激素配比的培养基对愈伤组织、增殖培养和生根培养的影响。[结果]消毒时闯以 10 min 最佳，花茎是莹苹组织培养的最佳外 植体;愈伤组织诱导的最佳培养基为:MS + 1. 5 mglL 6-BA +0.2 mglL IBA;在增殖培养中，MS + 1. 5 mglL 6-BA +0.2 mglL IBA 为最佳 增殖培养基。1I2MS +0. 3 mglL IBA 和1I2MS + 0.3 mglL NAA 培养基为4 个品种的最佳生根培养基。[结论]该方法建立了 4 个多用 途莹草品种的离体快繁体系，为莹萃的种质资源栽培提供了理论依据。 关键词 多用途萤草;组织培养;培养基;外植体;快繁体系 中图分类号 S188 文献标识码 A 文章编号 0517 -6611 (2014) 14 -04189 -03 S伽.dies on Tissue Culture and Rapid Propagation of the Four Multi-role Hemerocallis fulva CHU Bo-yan et aI (Hebei Institute of Forestry Science , Shijiazhuang , Hebei 05∞61) Abstract [ Objective ] To establish tissue culture and rapid propagation of tbe four multi-role Hemerocallis fu1va. [Method ] Witb tbe four multi-role H. ρdva as test materials , effects of disinfection time on different explants were studied , effects of culture medium with different hor- mone ration on tissue callus , proliferatiün and rooting culture were studied. [Result]ηle results showed that 10 min w臼 the best disinfection time , and the best explant w臼 scape; The best medium for callus was MS + 1. 5 mgl L6-BA +0. 2 mglL IBA; Shoot reproduction medium was MS+ 1. 5 mgl I..6-BA+0.2 mglLIBA;ηle best rooting medium was 1I2MS + 0.3 mglL IBA and 1I2MS + 0.3 mglL NAA. [ConclusionJ The tissue culture and rapid propagation of four multi-role He阳rocallis ρdva were established , which will provide theoretical basis for genn- plasm resource cultivation of Hemerocallis fu1va. Key words Multi-role Hemerocallis ρdva; Tissue culture; Culture medium; Explants; Rapid propa伊tlOn 宣草( Hemerocallis fu1va )为百合科( Lil