acupuncture on the blood flow of various organs measured simultaneously by colored microspheres in rats针灸在各种器官的血流量测量同时有色微球的老鼠.pdfVIP

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acupuncture on the blood flow of various organs measured simultaneously by colored microspheres in rats针灸在各种器官的血流量测量同时有色微球的老鼠.pdf

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acupuncture on the blood flow of various organs measured simultaneously by colored microspheres in rats针灸在各种器官的血流量测量同时有色微球的老鼠

Advance Access Publication 30 June 2007 eCAM 2009;6(1)77–83 doi:10.1093/ecam/nem068 Original Article Acupuncture on the Blood Flow of Various Organs Measured Simultaneously by Colored Microspheres in Rats Hiroyuki Tsuru1 and Kenji Kawakita2 1Department of Clinical Acupuncture and Moxibustion III, Meiji University of Oriental Medicine and 2Department of Physiology, Meiji University of Oriental Medicine, Hiyoshi-cho, Nantan – shi, Kyoto 629 - 0392, Japan We examined how acupuncture affected the blood flow of muscle, kidney, stomach, small intestine, brain, lung, heart, spleen and liver. Wistar rats anesthetized with urethane (n ¼ 27) were allocated into the control (n ¼ 10), ST-7 (Hsia-Kuan, n ¼ 10) and LI-4 (Hoku, n ¼ 7) groups. To measure organ blood flow, colored microspheres (CMS) were injected through a catheter positioned in the left ventricle and blood samples were drawn from the femoral artery. Yellow CMS (3.6–4.2 105 5 ) and blue CMS (6.0–6.9 10 ) were injected at intervals of about 30 min. An acupuncture needle (f 340 mm) was inserted into the left ST-7 point (left masseter muscle) or the right LI-4 point after the first sampling and left for about 30 min