agrobacterium-mediated gene transfer to cereal crop plants current protocols for barley, wheat, triticale, and maize农杆菌介导基因转移到谷类作物当前协议大麦、小麦、黑小麦、玉米.pdfVIP

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agrobacterium-mediated gene transfer to cereal crop plants current protocols for barley, wheat, triticale, and maize农杆菌介导基因转移到谷类作物当前协议大麦、小麦、黑小麦、玉米.pdf

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agrobacterium-mediated gene transfer to cereal crop plants current protocols for barley, wheat, triticale, and maize农杆菌介导基因转移到谷类作物当前协议大麦、小麦、黑小麦、玉米

Hindawi Publishing Corporation International Journal of Plant Genomics Volume 2009, Article ID 835608, 9 pages doi:10.1155/2009/835608 Research Article Agrobacterium -Mediated Gene Transfer to Cereal Crop Plants: Current Protocols for Barley, Wheat, Triticale, and Maize Goetz Hensel,1 Christine Kastner,1 Sylwia Oleszczuk,1, 2 Jan Riechen,1 and Jochen Kumlehn1 1 Plant Reproductive Biology, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, 06466 Gatersleben, Germany 2 Plant Breeding and Acclimatization Institute, Radzikow, 05-870 Blonie, Poland ´ Correspondence should be addressed to Goetz Hensel, hensel@ipk-gatersleben.de Received 8 April 2009; Accepted 17 April 2009 Recommended by Hikmet Budak The development of powerful “omics” technologies has enabled researchers to identify many genes of interest for which comprehensive functional analyses are highly desirable. However, the production of lines which ectopically express recombinant genes, or those in which endogenous genes are knocked down via stable transformation, remains a major bottleneck for the association between genetics and gene function in monocotyledonous crops. Methods of eﬀective DNA transfer into regenerable cells of immature embryos from cereals by means of Agrobacterium tumefaciens have been modiﬁed in a stepwise manner. The eﬀect of particular improvement measures has often not been signiﬁcantly evident, whereas their combined implementation has resulted in meaningful advances. Here, we provide updated protocols for the Agrobacterium -mediated generation of stably transgenic barley, wheat, triticale and maize. Based upon these methods, several hundred independ