allelic diversity of major histocompatibility complex class ii drb gene in indian cattle and buffalo等位基因的多样性主要组织相容性复合体ii级drb基因在印度牛和水牛.pdfVIP

SAGE-Hindawi Access to Research Molecular Biology International Volume 2011, Article ID 120176, 7 pages doi:10.4061/2011/120176 Research Article Allelic Diversity of Major Histocompatibility Complex Class II DRB Gene in Indian Cattle and Buffalo Sachinandan De,1 Raj Kumar Singh,2 and Biswajit Brahma3 1Animal Biotechnology Centre, National Dairy Research Institute, Karnal 1, Haryana 23001, India 2 National Research Centre on Equines, Sirsa Road, Hisar 1, Haryana 25001, India 3 KVK, SKUAST-Jammu, Bhaderwah, Jammu 182221, India Correspondence should be addressed to Biswajit Brahma, jitt77@ Received 20 September 2010; Accepted 6 January 2011 Academic Editor: Andrzej Kloczkowski Copyright © 2011 Sachinandan De et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The present study was conducted to study the diversity of MHC-DRB3 alleles in Indian cattle and buffalo breeds. Previously reported BoLA-DRB exon 2 alleles of Indian Zebu cattle, Bos taurus cattle, buffalo, sheep, and goats were analyzed for the identities and divergence among various allele sequences. Comparison of predicted amino acid residues of DRB3 exon 2 alleles with similar alleles from other ruminants revealed considerable congruence in amino acid substitution pattern. These alleles showed a high degree of nucleotide and amino acid polymorphism at positions forming peptide-binding regions. A higher rate of nonsynonymous substitution was detected at the peptide-binding regions, indicating that BoLA-DRB3 allelic sequence evolution was driven by positive selection. 1. Introduction