role of hoco in the control of peripheral circulation in humanshoco的作用在人类的末梢循环的控制.pdf

Hindawi Publishing Corporation International Journal of Hypertension Volume 2012, Article ID 236180, 4 pages doi:10.1155/2012/236180 Clinical Study Role of HO/CO in the Control of Peripheral Circulation in Humans David Sacerdoti, Despina Mania, Paola Pesce, Silvia Gaiani, Angelo Gatta, and Massimo Bolognesi Department of Clinical and Experimental Medicine, Clinica Medica 5, University of Padova, Via Giustiniani 2, 35100 Padova, Italy Correspondence should be addressed to David Sacerdoti, david.sacerdoti@unipd.it Received 16 November 2011; Accepted 7 December 2011 Academic Editor: David E. Stec Copyright © 2012 David Sacerdoti et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Experimental studies show that the heme oxygenase/carbon monoxide system (HO/CO) plays an important role in the homeostasis of circulation and in the pathophysiology of hypertension. No data are available on its role in the control of peripheral circulation in humans. We evaluated the effects of inhibition of HO with stannous mesoporphyrin IX (SnMP) (200 µM) locally administered by iontophoresis, on human skin blood flow, evaluated by laser-Doppler flowmetry, in the presence and absence of nitric oxide synthase (NOS) inhibition with L-NG-Nitroarginine methyl ester (L-NAME) (100 µM). We also evaluated the effect of HO inhibition on vasodilatation induced by acetylcholine (ACh) and vasoconstriction caused by noradrenaline (NA). SnMP and L- NAME caused a similar 20–25% decrease in skin flow. After nitric oxide (NO) inhibition with L-NAME, HO inhibition with SnMP caused a further 20% decrease in skin p