role of icam-1 in the aggregation and adhesion of human alveolar macrophages in response to tnf-α and inf-γicam-1在人类肺泡巨噬细胞的聚集和粘附在回应tnf-αinf-γ.pdf

Research Paper Mediators of Inflammation, 10, 309 –313 (2001) IN TR AC E LLUL AR adhesion molecule-1 (ICAM-1)-mediated Role of ICAM-1 in the aggregation cellÐ cell adhesion is thought to play an important role at sites of inflammation. Recent evidence sug- and adhesion of human alveolar gests that ICAM-1 surface expression on alveolar macrophages in response to TNF-a macrophages is increased in pulmonary sarcoidosis and that inflammatory granuloma formation is char- and INF-g acterized by the aggregation of macrophages. The present study shows that ICAM-1 expression is sig- nificantly elevated on alveolar macrophages from patients with sarcoidosis in response to tumor necro- Masahiro SasakiC A , Yuriko Namioka, Takefumi Ito, sis factor-a (TNF-a ) and interferon-g (INF-g ) com- Noriko Izumiyama, Shin Fukui, Akiko Watanabe, pared with healthy controls. Aggregation and adhe- Masayuki Kashima, Masaaki Sano, Takanobu Shioya sion were significantly increased in alveolar and Mamoru Miura macrophages treated with TNF-a and INF-g , and significantly inhibited in those pretreated with a monoclonal antibody to ICAM-1. Similarly, aggrega- tion and adhesion were inhibited in macrophages Second Department of Internal Medicine, Akita treated with heparin, which then exhibited a wide University School of Medicine, 1–1–1 Hondo, Akita, range of biological activities relevant to inflamma- 010, Japan tion. These results suggested that the surface expres- sion of ICAM-1 on alveolar macrophages in response to TNF- a and INF-g is important in mediating aggrega- tion and adhesion. Additionally, heparin may be CA Correspond